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pGEX-6p1-GST-ArcΔCTD

Plasmid

#119878

Purpose

Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

Depositor

Jason Shepherd

Article

Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

Insert

Activity Regulated Cytoskeletal Protein (Arc Rat)

Tags

GST

Expression

Bacterial

Mutation

Deletion of nt 832-1110

Promoter

tac

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD

Plasmid

#225702

Purpose

Lentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

H3.3 (K27M) (H3-3A, H3-3B Human)

Use

Lentiviral and Synthetic Biology

Tags

3xAID HA and T2A-mTurquoise

Expression

Mammalian

Mutation

K27M

Promoter

pEF1A

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC30A6_STOP

Plasmid

#161231

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A6 (SLC30A6 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCR4_miniFXN_7

Plasmid

#234360

Purpose

Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.

Depositor

Marek Napierala

Article

Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

Insert

FXN (FXN Human)

Use

Cloning

Tags

Flag Tag

Mutation

There is a partial deletion in the 5'UTR of …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF0361

Plasmid

#142255

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TCEB3 (ELOA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1.16

Plasmid

#212532

Purpose

Encodes SARS-CoV-2 variant XBB.1.16 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1.16 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG

Plasmid

#116774

Purpose

Lentiviral expression of PIK3CG

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

WT

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-888 USP7

Plasmid

#131248

Purpose

Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

Plasmid

#108693

Purpose

For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Inserts

RNASEH2B (RNASEH2B Human)
RNASEH2C (RNASEH2C Human)
RNASEH2A-D34A/D169A

Tags

GST

Expression

Bacterial

Mutation

Shine Dalgarno sequence upstream of start codon, …

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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TERT - R865C

Plasmid

#213926

Purpose

Expresses Mutant TERT R865C

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Mutant TERT R865C (TERT Human)

Use

Lentiviral

Tags

6x His Tag

Expression

Mammalian

Mutation

Arginine 865 changed to Cysteine

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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STT3A pEGFP-N2 (1051)

Plasmid

#62025

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-N-Myc_535-775 USP7

Plasmid

#131247

Purpose

Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 EG.5.1

Plasmid

#212454

Purpose

Encodes SARS-CoV-2 variant EG.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike EG.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Jam2-Fc-His

Plasmid

#72079

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB

Plasmid

#212521

Purpose

Encodes SARS-CoV-2 variant XBB Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCIG-VP16–RAR-alpha

Plasmid

#80434

Purpose

Expression of constitutively active human RAR-alpha in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Retinoic acid receptor alpha (RARA Human)

Tags

EGFP and VP16

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pSS02:cyt-Peredox-mCherry_DS

Plasmid

#161747

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry_DS

Expression

Plant

Mutation

amino acid substitution D115S in first T-Rex doma…

Promoter

Ubiquitin 10

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPL001

Plasmid

#184750

Purpose

Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

Depositor

Jef Boeke

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

pEF1a-eGFP-T2A-BSD-bGHpA

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-E1051K

Plasmid

#116553

Purpose

Lentiviral expression of PIK3CB E1051K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Mutation

E1051K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2_RNAmut

Plasmid

#73539

Purpose

Inducible lentiviral expression of Ago2_RNAmut

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Small RNA binding mutant PAZ domain Y311A and F31…

Promoter

TRE promoter, Tet ON

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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