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pMCSG7-dNmeCas9

Plasmid

#71475

Purpose

bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-Inactive-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A H588A

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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SiT-EGFP

Plasmid

#166943

Purpose

mammalian expression of N' Sialyl Transferase tagged with EGFP

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Cell. 2008 Jun 13;133(6):1055-67. doi: 1

Insert

Sialyl Transferase N-terminus

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETB-ro2GFP

Plasmid

#82366

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro2GFP

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/S65T/Q80R/S147C/Q204C

Promoter

T7

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-PP7-boxB

Plasmid

#75393

Purpose

sgRNA1-PP7-boxB

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7-boxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pHR Clover-VISH2

Plasmid

#188629

Purpose

Fluorescent reporter for SLP76/VISH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

VISH2

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pERB217

Plasmid

#61500

Purpose

Const. Halotag-GFP-Mito + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Mito
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2-E451A

Plasmid

#73673

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
DGK iota

Plasmid

#35409

Depositor

Robert Lefkowitz, Stephen Prescott

Article

Nelson et al Science. 2007 Feb 2;315(5812):663-6.

Insert

DGK iota (DGKI Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSC101-GFPmut2-mScarlet-I TAG

Plasmid

#208188

Purpose

nonsense mistranslation, TAG stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAG*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGA

Plasmid

#208189

Purpose

nonsense mistranslation, TGA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGATC

Plasmid

#208190

Purpose

nonsense mistranslation, TGA stop variant 2

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7TCT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I T7

Plasmid

#208191

Purpose

frameshift mistranslation, T7

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6TTTTTTT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A5

Plasmid

#208192

Purpose

frameshift mistranslation, A5

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A4

Plasmid

#208193

Purpose

frameshift mistranslation, A4

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A6T

Plasmid

#208194

Purpose

frameshift mistranslation, A6T

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAAAAT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I C3T

Plasmid

#208195

Purpose

frameshift mistranslation, C3T

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6CCCT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I 6Trp7Ser

Plasmid

#208186

Purpose

mistranslation GFP and mScarlet-I positive control with Trp insertion

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6Trp7Ser

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4um200-ns

Plasmid

#67696

Purpose

middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4um200

Tags

HA

Mutation

um200, no stop codon; N1056S

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA3 TKIT donor

Plasmid

#169425

Purpose

Donor DNA for TKIT knockin of SEP to GluA3 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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