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pMTKM30

Plasmid

#233502

Purpose

Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

TDH3-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM31

Plasmid

#233503

Purpose

Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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p40phox-PX (1-144)

Plasmid

#119123

Purpose

Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

p40phox-PX (1-144)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL CAGpNFlag BAF155 ires GFP

Plasmid

#24561

Depositor

Jerry Crabtree

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

Insert

BAF155 (Smarcc1 Mouse)

Use

Lentiviral

Tags

Flag

Available Since

July 16, 2010

Availability

Academic Institutions and Nonprofits only
FLAG_hPPP1R15B_4-713_mCherry_UK1298

Plasmid

#80707

Purpose

mammalian expression N-term FLAG and C-term mCherry hPPP1R15B (CReP 4-713)

Depositor

David Ron

Article

Chen et al Elife. 2015 Mar 16;4. doi: 10.7554/eLife

Insert

human PPP1R15B (PPP1R15B Human)

Tags

mCherry

Expression

Mammalian

Mutation

CDS 4-713 no stop codon

Promoter

CMV

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pN256

Plasmid

#188472

Purpose

scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

eGFP-WPRE-DAISY-4T

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSBK.079

Plasmid

#187218

Purpose

Expresses Eco1 RT and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 Reverse Transcriptase
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23115 and T7/lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD4

Plasmid

#20186

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 4 Intracellular Domain (Notch4 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 4 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only
Ccas9LacZ

Plasmid

#113083

Purpose

Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

CRISPR

Expression

Worm

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF-G1mCNPRS

Plasmid

#174718

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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UKNP3.1.2

Plasmid

#98215

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-EGFP-LDLR(Ct)

Plasmid

#213402

Purpose

douple floxed, myristoylation (myr) site for membrane targeting and EGFP

Depositor

Katrin Willig

Article

Wegner et al Elife. 2022 Feb 23;11:e73603. doi: 10.75

Insert

EGFP

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMTKM07

Plasmid

#233479

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEM3_Euk_2NLS-Flex-Cas12a (LbCas12a)-2NLS

Plasmid

#244838

Purpose

Mammalian expression of Flex-Cas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP8H

Plasmid

#188062

Purpose

PROSS-designed versatile peroxidase 8H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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UKNP4.2.2

Plasmid

#98268

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F265-OKT3

Plasmid

#190679

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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UKNP5.2.1

Plasmid

#98385

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.10.1

Plasmid

#97376

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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