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Rtn3LΔ6LIR-mNeonGreen

Plasmid

#169036

Purpose

Express human Rtn3L with 6x LC3-interacting regions mutated in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

Changed following LC3-interacting regions of Rtn3…

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG17-mCherry-HOTag3

Plasmid

#190024

Purpose

Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

LaG17 CluMPS

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRMC2-msfGFP

Plasmid

#194913

Purpose

Tetracycline inducible expression of msfGFP in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno Sequence followed by monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Expression

Bacterial

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF1A

Plasmid

#172206

Purpose

Expresses KIF1A tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF1A (Kif1a Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pmEmerald-ERGIC-53

Plasmid

#170717

Purpose

Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

ERGIC-53 (LMAN1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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8138-E02

Plasmid

#225659

Purpose

Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)

Depositor

Dominic Esposito

Article

Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

Insert

SORE6

Use

Gateway entry clone

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-METTL17-8XHIS

Plasmid

#215705

Purpose

Express METTL17-8XHIS in C41(DE3) cells

Depositor

Vamsi Mootha

Article

Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

Insert

METTL17 (METTL17 Human)

Tags

HIS

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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T-2A-EGFP-PGK-Puro

Plasmid

#83344

Purpose

Donor vector to knocin EGFP into human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

Human Brachyury knockin homology arms (TBXT Human)

Tags

2A-EGFP

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro p53 shRNA 1

Plasmid

#10671

Depositor

William Hahn

Article

Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

Insert

p53 shRNA (TP53 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pFUGW-FerH-ffLuc2-eGFP

Plasmid

#71393

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

FerH-ffLuc2-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

FerH

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

Plasmid

#126705

Purpose

donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

GFP

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSF3-microID

Plasmid

#172880

Purpose

expresses microID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA331Bb

Plasmid

#78269

Purpose

pSEVA331 with Biobrick multiple cloning site

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Type

Empty backbone

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2

Plasmid

#108940

Purpose

Mammalian expression of mRIN2

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLUT7 HA-GLI1

Plasmid

#62970

Purpose

Expresses HA-tagged Gli1

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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IRE1 alpha-pcDNA3.EGFP

Plasmid

#13009

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Expression

Mammalian

Available Since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PHD3

Plasmid

#21402

Depositor

Eric Metzen

Article

Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

Insert

EGLN3 (EGLN3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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