We narrowed to 13,836 results for: ckb
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Plasmid#62891PurposeTrojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneAvailable SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only
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pHLmMBP-6
Plasmid#72346PurposeMammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianPromoterCMV enhancer + chicken beta-actin promoterAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectPromoterpolyhedrinAvailable SinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGWB408
Plasmid#74802PurposeGateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDF0336 pAAV EFS-HvsCas7-11 for dual vector
Plasmid#172515PurposeEncodes HvsCas7-11 mammalian expression casette in AAV backboneDepositorInsertpAAV EFS-HvsCas7-11 for dual vector
UseAAVAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635
Plasmid#91918PurposeExpresses LbfCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLbfCas13a and guide expression cassette
ExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneTagsAsLOV2 wt and mCherryExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3 Pro E2
Plasmid#48749PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH2
Plasmid#168978PurposeAMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…Available SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneExpressionBacterialPromoterTetracycline promoterAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
ExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB120
Plasmid#68574PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-mB2M
Plasmid#154091PurposeSelf-Cutting and Integrating CRISPR backbone targeting murine B2MDepositorInsertsgRNA and BAIT targeting murine B2M locus
UseCRISPRExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE666
Plasmid#153231PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS
Plasmid#199348PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-TEVExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB545
Plasmid#74879PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNSA-ARS-CRISPR-BlastR
Plasmid#101009PurposeExpresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.DepositorTypeEmpty backboneUseAlgae, nannochloropsisAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH4
Plasmid#168980PurposeAMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…Available SinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS
Plasmid#199350PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOstarExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only