We narrowed to 2,572 results for: GEM
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Plasmid#85792PurposeP. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLIDepositorInsertKelch13
UseCre/Lox; P. falciparum genomic integrationTags2xFKBP, GFPPromoternoneAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH6_new
Plasmid#105866Purposemore efficient version of pFH6; Subcloning of any sgRNA via BbsI siteDepositorInsertU6-26p::sgRNA scaffold
UseCRISPR and Synthetic Biology; SubcloningExpressionPlantAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBFP (GB0025)
Plasmid#68193PurposeProvides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid partDepositorInsertBFP coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hMed25
Plasmid#206958PurposeThe ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.DepositorInsertHomo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PROM5_35Sx2
Plasmid#136112PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35Sx2 (CaMV)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM5_35S
Plasmid#136111PurposeStrong promoter that drives expression everywhere in Mp gemmae but much weaker in the notchesDepositorInsertPROM_35S (CaMV)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-NQO1
Plasmid#136766PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Nqo1DepositorInsertNqo1 (Nqo1 Mouse)
UseIn situ probeMutationISH Probe insert, V184A- Please see depositor co…Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-mRFP
Plasmid#128126PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterpBen (benzoate responsive promoter)Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
StrepII-tag
Plasmid#55181PurposeStreptactin-tagDepositorInsertStrepII tag
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFP.R275
Plasmid#138224PurposeExpresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCngA2-ISH
Plasmid#15855DepositorInsertCyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)
UseCloning vectorAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPnos (GB0072)
Plasmid#68166PurposeProvides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid partDepositorInsertNos promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDsRed (GB0100)
Plasmid#68202PurposeProvides the CDS of DsRed as a level 0 GoldenBraid partDepositorInsertDsRed coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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His10-tag
Plasmid#55179Purpose10xHis-tagDepositorInsertHis10
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
7iBi89 (rat fibronectin)
Plasmid#14065DepositorInsertRat Fibronectin 7iBi89 (Fn1 Rat)
ExpressionMammalianMutationInsert was cut out of pGEM3 with SalI and SacI an…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
cMyc-tag
Plasmid#55183PurposecMyc tagDepositorInsertcMyc tag
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYFP (GB0053)
Plasmid#68197PurposeProvides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid partDepositorInsertYFP coding region
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-BenR
Plasmid#128125PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing BenR transcription factor gene under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP and BenR
UseSynthetic BiologyExpressionBacterialPromoterpBen and J23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII
Plasmid#80864PurposePhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G,P
ExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
ExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBW216J106-Amp32C
Plasmid#78651PurposepSB3K3 carrying J106-30hrpR-32hrpS-t-hrpL-30gfp-tTDepositorInsertJ106-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyPromotersee insertAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFL-TK-AID3'UTR
Plasmid#19986DepositorInsertAID 3' UTR (Aicda Mouse)
ExpressionMammalianAvailable SinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pO-macs-ISH
Plasmid#15856DepositorInsertOlfactory specific medium-chain acyl CoA synthetase (Acsm4 Mouse)
UseCloning vectorAvailable SinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcFN-C (chick fibronectin)
Plasmid#14063DepositorInsertChicken Fibronectin (FN1 Chicken)
ExpressionMammalianMutationDraI - EcoRI fragment inserted in HincII-EcoRI si…Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP
Plasmid#72954PurposeLevel 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDEDepositorInsertLevel 2 - A site
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW214J115-Amp32C
Plasmid#78649PurposepSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ115-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyPromotersee insertAvailable SinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-CocE
Plasmid#128129PurposeExpressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032DepositorInsertCocE
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only