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Plasmid#172717PurposeDelivery of guide RNA for prime editingDepositorInsertJ23119-gRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIM24-GUS
Plasmid#67882PurposePlant Gene Expression Vector pSIM24 containing the GUS (Beta-glucuronidase) reporter geneDepositorInsertuidA
UseTagsExpressionPlantMutationT120APromoterM24Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pG108 - K
Plasmid#178039PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markersDepositorInsertKanamycin resistance
UseTagsExpressionBacterialMutationErmAM has been replaced with Kanamycin for select…PromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEN-luxCDABE
Plasmid#44918DepositorInsertsigma 70 promoter
UseLuciferaseTagslux transcriptional fusionExpressionMutationPromoterem7Available sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR58.6
Plasmid#63176PurposeExpresses CcaR constitutively and sfGFP under the PcpcG2-172 promoterDepositorInsertsCcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKL10-mRuby-iATPSnFR1.0 bb118
Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
UseTagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMF19
Plasmid#113662PurposeContaining the wbbL (rhamnosyltransferase) gene cloned for mutant complementationDepositorInsertwbbL
UseTagsExpressionMutationPromoterPtacAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-HA-BirAonly
Plasmid#232584PurposeExpress HA-BirADepositorInsertBirA
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
psfaMs-Tn5
Plasmid#183637PurposeExpresses anti-mouse nanobody fused with Tn5DepositorInsertanti mouse secondary nanobody fused to Tn5
UseTagsanti-mouse nanobodyExpressionBacterialMutationPromoterAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
MscL(G22S)61-cpGFP-ERexp
Plasmid#195311PurposeFluorescent reporter/biosensor for membrane tensionDepositorInsertMscL(G22S)61-cpGFP-ERexp
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLII12Pglu-700uDelta6
Plasmid#17866DepositorInsertFLII12Pglu-700uDelta6
UseTagsCitrineExpressionMammalianMutation700uDelta6, ECFP insert in mglBPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-XNS
Plasmid#196655PurposePromega's psiCHECK-2 Dual-Luciferase Reporter vector modified to allow the insertion of the target in opposite directionsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSHDY-Prha-mVenus_rhaS
Plasmid#137662PurposeConjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.DepositorInsertPrha:mVenus
UseTagsExpressionBacterialMutationrhaS (Arg218Leu)PromoterPrhaAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-strong
Plasmid#108313PurposeGFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.DepositorInsertGreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterpFAB4026Available sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Sp
Plasmid#174726PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCYP64
Plasmid#212897PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationP450BM3 MERO1 (V78A, A82G, F87V, P142S, T175I, A1…PromoterPcymRCAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Ts-His6
Plasmid#224371PurposeExpress EF-Ts protein for his-tagged purificationDepositorInsertEF-Ts (tsf E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-G-His8
Plasmid#224369PurposeExpress EF-G protein for his-tagged purificationDepositorInsertEF-G (fusA E. coli)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Tu-His8
Plasmid#224370PurposeExpress EF-Tu protein for his-tagged purificationDepositorInsertEF-Tu (tufB E. coli)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-PheRSa-PheRSb
Plasmid#224364PurposeExpress PheRS protein for his-tagged purificationDepositorInsertPheRS (pheS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-AlaRS
Plasmid#224362PurposeExpress AlaRS protein for his-tagged purificationDepositorInsertAlaRS (alaS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAJM.773
Plasmid#108527PurposeVanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.DepositorInsertVanRAM + PVanCC-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNatB (pACYCduet-naa20-naa25)
Plasmid#53613PurposeAllows expression of the fission yeast NatB complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-mNeon-TurboID
Plasmid#186610PurposeThird generation lentiviral vector expressing mNeonGreen fused to V5-TurboIDDepositorInsertmNeon-V5-TurboID
UseLentiviralTagsmNeonGreenExpressionMammalianMutationFor reference see Branon et al., 2018PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL278_P23-DsRed-Express2
Plasmid#121505PurposeE. coli-streptococcal shuttle vector that expresses rfpDepositorInsertDsRed-Express2
UseE. coli streptococcal shuttle vectorTagsExpressionMutationPromoterP23Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-BirA-ER
Plasmid#20856DepositorInsertBirA-ER
UseTagsExpressionMammalianMutationC-terminal ER targeting sequence (KDEL)PromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBEL1994
Plasmid#139898PurposeExpression plasmid for LetB PLL2 deletion (without TM region)DepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; delta229-239, D228G, L240G)-6xHisPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-Hyg
Plasmid#210261PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
p005-RFP-strong
Plasmid#108317PurposeSynthetic RFP based on Corynactis spp under strong synthetic constitutive promoter pFAB4005; kanamycin resistant.DepositorInsertRFP under controlf of strong synthetic constitutive promoter pFAB4005
UseTagsExpressionBacterialMutationPromoterpFAB4005Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato
Plasmid#60658PurposeFlp-dependent expression of mNaChBac-T2A-tdTomatoDepositorHas ServiceAAV8InsertFLEX-mNaChBac-T2A-tdTomato
UseAAVTagsExpressionMutationPromoterEF1αAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMO9075
Plasmid#117481Purposeempty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecRDepositorInsertAph(3')-IIa promoter
UseTagsExpressionBacterialMutationPromoterAph(3')-IIa promoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbE1k-DDC
Plasmid#71723Purposedopa decarboxylase from pigDepositorInsertdopa decarboxylase
UseTagsExpressionMutationPromoterpTrcAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA3442
Plasmid#184852PurposeRetroviral vector encoding puromycin resistance and Cre recombinaseDepositorInsertCre
UseCre/Lox and RetroviralTagsMyc-tagExpressionMammalianMutationPromoterLTRAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPH6
Plasmid#40315DepositorInsertPeptide:N-glycosidase F
UseTags6xHis and OmpA3ExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBacMut-T2A-tdTomato
Plasmid#60660PurposeFlp-dependent expression of mNaChBacMut-T2A-tdTomatoDepositorInsertFLEX-mNaChBacMut-T2A-tdTomato
UseAAVTagsExpressionMutationE191KPromoterEF1αAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-NDK
Plasmid#124136PurposeBacterial expression plasmid for PURE system componentsDepositorInsertNucleotide diphosphate kinase (ndk Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
6his-WT gusA-pET28a+
Plasmid#61156PurposeN -terminal His tagged wild-type GUS expression vectorDepositorInsertgusA
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKIKOarsBCm
Plasmid#46763PurposeE.coli genomic integration at arsBDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLTS
Plasmid#59386PurposeExpresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.DepositorTypeEmpty backboneUseGenome engineeringTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
UseTagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-AqpZ-ALFA
Plasmid#212310PurposeExpression of AqpZ with ALFA tagDepositorInsertAquaporin Z
UseTagsALFA tag and Strep-Tag IIExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ykgMO-IGS-sfGFP
Plasmid#207231PurposeCalprotectin sensing promoter with sfGFP reporterDepositorInsertykgMO-IGS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas12f1
Plasmid#171182PurposeConstitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1DepositorInsertCas12f1
UseCRISPRTagsExpressionBacterialMutationPromotercas9 promoter from Streptococcus pyogenesAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKIKOlacZKm
Plasmid#46767PurposeE.coli genomic integration at lacZDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only