We narrowed to 21,797 results for: Sis

1. pUCSh8-2300

   Plasmid

   #170986

   Purpose

   Encodes sgRNA target 2300 and Hygromycin resistance transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMA3998rc

   Plasmid

   #184851

   Purpose

   A retroviral vector with blasticidin resistance for cloning inserts between loxP sites

   Depositor

   Mikhail Alexeyev

   Article

   Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

   Type

   Empty backbone

   Use

   Cre/Lox and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMA5132

   Plasmid

   #184858

   Purpose

   Retroviral vector, hygromycin resistance hTFAM expression

   Depositor

   Mikhail Alexeyev

   Article

   Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

   Insert

   TFAM human
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPQD0KN0025

   Plasmid

   #162283

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSKAP

   Plasmid

   #122702

   Purpose

   Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

   Depositor

   Travis Bourret

   Article

   Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCVD630

   Plasmid

   #66163

   Purpose

   cloning of ace gene

   Depositor

   James Kaper

   Article

   Trucksis et al Proc Natl Acad Sci U S A. 1993 Jun 1;90(

   Insert

   ace
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSAM_Ec

   Plasmid

   #102939

   Purpose

   Transposon mutagenesis and Tn-seq

   Depositor

   Matthew Mulvey

   Article

   Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

   Insert

   mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCBL101-RUBY

   Plasmid

   #199723

   Purpose

   Expresses betalain biosynthesis genes under CaMV 35S promoter

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   35S-RUBY
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. At2S3:RUBY

   Plasmid

   #160906

   Purpose

   RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and At2S3 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   At2S3

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGGZ001

    Plasmid

    #48868

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sox10:mCherry

    Plasmid

    #98695

    Purpose

    I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    mCherry
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNC-Green-LUC

    Plasmid

    #193438

    Purpose

    Nimble cloning-compatible vector for the analysis of promoter activity in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTORGm43GWY

    Plasmid

    #112906

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGGZ003

    Plasmid

    #48869

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHA-PUMA

    Plasmid

    #16588

    Depositor

    Bert Vogelstein

    Article

    Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

    Insert

    PUMA (BBC3 Human)
    

    Use

    Tags

    2x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUCSh5vio-2300

    Plasmid

    #170987

    Purpose

    Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300 violacein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placI

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTORGm43GWR

    Plasmid

    #112905

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTORGm43GWC

    Plasmid

    #112903

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTORGm43GWF

    Plasmid

    #112904

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

    Type

    Empty backbone

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHG4

    Plasmid

    #188252

    Purpose

    Integrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selection

    Depositor

    Hong Gao, Maggie Smith

    Article

    Gao et al BMC Biotechnol. 2019 Jun 4;19(1):32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSP64-Lifeact-GFP

    Plasmid

    #135601

    Purpose

    Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    Lifeact-GFP
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGGY001

    Plasmid

    #48866

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGGY003

    Plasmid

    #48867

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEPQDKN0248

    Plasmid

    #162313

    Purpose

    Reporter plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-sfGFP-HiBiT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKI1.1

    Plasmid

    #85807

    Purpose

    CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS426-GFP-PLCdelta-PH

    Plasmid

    #71375

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    PLCdelta-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain

    Promoter

    Available since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pMXLK_aFb

    Plasmid

    #114230

    Purpose

    POC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNC-121-Pro

    Plasmid

    #193437

    Purpose

    Nimble cloning-compatible vector for the analysis of promoter activity in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSP64-membrane-mCherry

    Plasmid

    #135598

    Purpose

    Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    membrane-mCherry
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pZCAN

    Plasmid

    #129115

    Purpose

    Z. rouxii centromeric vector containing ClonNAT resistance

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYCTK028

    Plasmid

    #176732

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    ste2Tp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBS-TarHom-Tol2-TetR-cryaa-YFP

    Plasmid

    #73202

    Purpose

    BAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker Citrine

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    YFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pZEN

    Plasmid

    #129113

    Purpose

    Z. rouxii episomal vector containing ClonNAT resistance

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMXLK_dFq

    Plasmid

    #114237

    Purpose

    POC12377, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type d-q

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMXLK_pFa

    Plasmid

    #114229

    Purpose

    POC12367, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type p-a

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pICH50581

    Plasmid

    #50256

    Depositor

    Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, act2 (AT3G18780, Arabidopsis thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXLC_pFa

    Plasmid

    #114219

    Purpose

    POC12355, MetClo assembly vector with p15A replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFLLFLN

    Plasmid

    #40030

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    FRT-Lox5171-Lox2272-FRT-loxP
    Neo
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    None and SV40

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pME-nEGFP

    Plasmid

    #138363

    Purpose

    Gateway compatible middle entry clone contains nuclear EGFP sequences.

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    nEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMXLP_pFa

    Plasmid

    #114239

    Purpose

    POC12379, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type p-a

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCR2.1-ZFpparg

    Plasmid

    #44151

    Purpose

    This is intended for use in riboprobe synthesis, not for cloning.

    Depositor

    John Rawls

    Article

    Flynn EJ et al J Lipid Res. 2009 Aug;50(8):1641-52. doi

    Insert

    Peroxisome proliferator-activated receptor gamma (pparg Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

42. FIRESFLIP-PBCS-E670

    Plasmid

    #228701

    Purpose

    Sensor cassette FIRE2, sensor in backwards orientation, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE2 SENSOR
    

    Use

    Synthetic Biology and Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCBL102-RUBY

    Plasmid

    #210379

    Purpose

    Expresses betalain biosynthesis genes under CaMV 35S promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    35S-RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCfuNca-Mito

    Plasmid

    #190819

    Purpose

    Mitochondria targeted GFP for diatoms

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom mitochondrial targeted GFP
    Bleomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUCSh2-2300

    Plasmid

    #170984

    Purpose

    Encodes sgRNA target 2300 and Kanamycin resistance transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pUCSh3-2300

    Plasmid

    #170985

    Purpose

    Encodes sgRNA target 2300 and Tetracycline resistance transposon.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    Sh-CAST sgRNA 2300
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. MiniSOG-SpAGS-GFP

    Plasmid

    #185581

    Purpose

    for CALI

    Depositor

    Mamiko Yajima

    Article

    Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

    Insert

    miniSOG-SpAGS
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pYCTK017

    Plasmid

    #176721

    Purpose

    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Tetrapisispora phaffii (sender part).

    Depositor

    Victor Sourjik

    Article

    Krink et al bioRxiv 2021

    Insert

    mfalpha1Tp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon-optimized for expression in S. cerevisiae

    Promoter

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEPQDKN0329

    Plasmid

    #162315

    Purpose

    TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-NET-TEVprotease-HiBiT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEPQD0KN0244

    Plasmid

    #162282

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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