Skip to main content
Addgene
Showing: 2801 - 2850 of 6422 results

  3. RhoA sensor (LSS-SGFP2-mCherry)

    Plasmid
    #112948
    Purpose
    RhoA FRET biosensor
    Depositor
    Dorus Gadella
    Insert
    Rhoa sensor
    Use
    Tags
    LSS-SGFP2 and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112948-plasmid-map-sequence-id-220190

    Enlarge

  10. pAAV-anti-mCherry(LaM8) PAGER(TF)

    Plasmid
    #229999
    Purpose
    Expresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229999-plasmid-map-sequence-id-457197

    Enlarge

  11. pAAV-anti-mCherry(LaM6) PAGER(TF)

    Plasmid
    #229998
    Purpose
    Expresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
    Depositor
    Alice Ting
    Article
    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-
    Insert
    IL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    V360A/R361A on KORD
    Promoter
    CMV
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229998-plasmid-map-sequence-id-457195

    Enlarge

  18. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129027-plasmid-map-sequence-id-256129

    Enlarge

  19. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid
    #192438
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    Use
    Tags
    Full-length LAMP1 and mCherry
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192438-plasmid-map-sequence-id-379134

    Enlarge

  24. pCDH-SFFV-GLuc-CreERT2-mCherry

    Plasmid
    #178185
    Purpose
    the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.
    Depositor
    Irmela Jeremias
    Article
    Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:
    Inserts
    GLuc
    CreERT2
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    178185-plasmid-map-sequence-id-351314

    Enlarge

  27. pTetON-DHFRdd-SpvB; CMV-mCherry

    Plasmid
    #89463
    Purpose
    Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)
    Depositor
    Brad Zuchero
    Article
    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n
    Insert
    DeAct-SpvB (spvB Salmonella enterica)
    Use
    Tags
    E. coli DHFRdd destabilization domain and mCherry…
    Expression
    Mammalian
    Mutation
    Truncation spanning SpvB amino acids 375-591 (mon…
    Promoter
    TRE3G
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89463-plasmid-map-sequence-id-176808

    Enlarge

  28. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid
    #50458
    Purpose
    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    rM3D-mCherry
    Use
    AAV
    Tags
    HA and mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50458-plasmid-map-sequence-id-162264

    Enlarge

  37. pKK-RNAi-nucEGFPmiR-TEV-mCherry

    Plasmid
    #105812
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105812-plasmid-map-sequence-id-203423

    Enlarge

  40. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid
    #35498
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35498-plasmid-map-sequence-id-199355

    Enlarge

  41. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid
    #112157
    Purpose
    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.
    Depositor
    Ben Giepmans
    Article
    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261
    Insert
    anti-GFP FLIPPER-body
    Use
    Tags
    His and Thrombin cleavage site
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    112157-plasmid-map-sequence-id-213698

    Enlarge

  42. pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)

    Plasmid
    #107939
    Purpose
    pie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.
    Depositor
    Heng-Chi Lee
    Article
    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d
    Insert
    mCherry(dpiRNA)::ANI-1(AHPH)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    pie-1
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107939-plasmid-map-sequence-id-207466

    Enlarge

Showing: 2801 - 2850 of 6422 results