We narrowed to 2,675 results for: CCS

Showing: 1121 - 1140 of 2675 results

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pFL-SV40-Rac1 5'-UTR

Plasmid

#115354

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

Rac1 5'-UTR (RAC1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pX330_rActb sgRNA / hSpCas9

Plasmid

#172833

Purpose

Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP1274

Plasmid

#119266

Purpose

B. subtilis deltaTn7 site and flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

B. subtilis deltaTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1263

Plasmid

#119264

Purpose

B. subtilis attTn7 site and flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

B. subtilis attTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_rSyn1 sgRNA / hSpCas9

Plasmid

#172840

Purpose

Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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MAP3K7CL_pCSdest

Plasmid

#53808

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MAP3K7CL (C21orf7 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
EZH2-TALEN_Right_No_FLAG

Plasmid

#79900

Purpose

Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Right_No_FLAG (EZH2 Human)

Use

TALEN

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-GNB2 5'-UTR

Plasmid

#115355

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

GNB2 5'-UTR (GNB2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pY036_ATP1A1_G5

Plasmid

#86618

Purpose

Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G5 crRNA + AsCpf1-3xHA

Use

CRISPR; Co-selection via hdr using ouabain

Tags

3xHA

Expression

Mammalian

Promoter

CBh

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-RG-mycStrep

Plasmid

#31840

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

R95G

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-103)

Plasmid

#60357

Purpose

Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-103) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains amino acids 7-103 of Msi1

Promoter

T7

Available Since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)R61E

Plasmid

#60353

Purpose

R61E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R61E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pLOC-D133p53a

Plasmid

#241916

Purpose

Lentiviral vector driving a p53 isoform delta133p53alpha. Stable expression in cultured human cells and possibly cells of other species.

Depositor

Curtis Harris

Article

Ungerleider et al Neuroscience. 2022 Aug 21;498:190-202. d

Insert

Delta133p53alpha (TP53 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-N Terminus (pGEX4T1)

Plasmid

#238422

Purpose

Expresses GRP78 N terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-Delta KDEL (pGEX4T1)

Plasmid

#238424

Purpose

Expresses GRP78 Delta KDEL fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GST-GRP78-C Terminus (pGEX4T1)

Plasmid

#238423

Purpose

Expresses GRP78 C Terminus fused to the GST Tag

Depositor

Amy Shiu Lee

Article

Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3

Plasmid

#233425

Purpose

IVT template for LIDAR reporter mRNA; mCherry as marker, rtetR-NZF as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0216 CMVTO-hIL6Ra_M4-rens-hIL6-rens-IL6Rb

Plasmid

#231194

Purpose

Multi-cageIL-6

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

hIL6Ra_M4-rens-hIL6-rens-IL6Rb (IL6 Human)

Expression

Mammalian

Mutation

A228D, N230D, H280S, D281V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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