We narrowed to 13,712 results for: Cas9

1. AAV-nEF-dCas9

   Plasmid

   #106430

   Purpose

   Expresses SpdCas9 from the nEF promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   nEF-dCas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAaePUb::Cas9-2A-Neo

   Plasmid

   #176680

   Purpose

   Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
   

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Drosophila codon optimized

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330a dCas9-VP64

   Plasmid

   #92363

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-dCas9-G9a-Hygro

   Plasmid

   #104408

   Purpose

   transient expression of dCas9-G9a fusion protein

   Depositor

   Stephan Beck

   Insert

   G9a (EHMT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-dCas9-trCIB1

   Plasmid

   #64119

   Purpose

   Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   dCas9-trCIB1 fusion
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   dCas9 (D10A H840A, catalytically inactive), trCIB…

   Promoter

   CMV

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCF140_Gag-SpyCas9-NLS

   Plasmid

   #225959

   Purpose

   CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   CMV-Intron-Gag-SpyCas9-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-CBE6a-IVT

   Plasmid

   #215833

   Purpose

   Template for in vitro transcription of CBE6a (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-nSpCas9-APOBEC3A

   Plasmid

   #209040

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC3A-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. 138-dCas9-Dnmt3a

   Plasmid

   #84570

   Purpose

   PiggyBac transposon system construct with dCas9-Dnmt3a

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid

    #102851

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. G1333 DddAtox-C–dSpCas9

    Plasmid

    #157834

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. spCas9-NLS-MAV

    Plasmid

    #124212

    Purpose

    SpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)

    Depositor

    Uri David Akavia

    Article

    Roche et al CRISPR J. 2018 Dec;1:414-430. doi: 10.10

    Insert

    cas9
    

    Use

    CRISPR

    Tags

    His6, MBP, TEV Cleavage site, and two copies of S…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. dCas9-CRY2Clust-BRD4ΔN

    Plasmid

    #200968

    Purpose

    induction of phase-separated condensate mediated by BRD4dN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    BRD4dN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pdCas9-tdTomato-VPR

    Plasmid

    #232632

    Purpose

    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer

    Depositor

    Karsten Rippe

    Insert

    dCas9-tdTomato-VPR
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459V2.0-SpCas9-HF1

    Plasmid

    #108293

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-dCas9-D3A

    Plasmid

    #78256

    Purpose

    Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

    Depositor

    Grant Challen

    Article

    McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

    Insert

    DNMT3A CD aa 598-912 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. HiFi dCas9 VPR

    Plasmid

    #188510

    Purpose

    Expresses FLAG tagged HiFi dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAH-CTX1-rhadCas9

    Plasmid

    #129391

    Purpose

    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Burkholderia cenocepacia codon-optimized dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Entire dCas9 codon optimized for the GC-rich B. c…

    Promoter

    PrhaBAD

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

    Plasmid

    #110302

    Purpose

    Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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