We narrowed to 2,335 results for: D10
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Plasmid#225149PurposeGateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editingDepositorInsertLoA3GX1-zCas9(D10A)-UGI
ExpressionPlantAvailable SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 R3 W
Plasmid#231876PurposeBacterial expression of N-terminally 6His tagged Gcn4 R3 WDepositorInsertGcn4 R3 W
Tags6xHis, TEV cleavage siteExpressionBacterialMutationD103R, N126W, D133R, K143RPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMO1170
Plasmid#232073PurposeExpresses Adenine Base Editor for Mammalian CellsDepositorInsertAdenine Base Editor
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1-alpha core promoterAvailable SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Yeast_Target-AID2S
Plasmid#188647PurposeExpressing Target-AID2S in yeastsDepositorInsertnCas9-AID2S-UGI
TagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEAF1.2.2-gDNA
Plasmid#113799PurposeCRISPR plasmid for expression of Cas9 nicakse (D10A) and gRNA targeting human transcription factor DEAF1DepositorAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEAF1.2.1-gDNA
Plasmid#112449PurposeCRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAL3591
Plasmid#47310DepositorInsertZebrafishCommunity-yjefn3-right (si:ch211-212d10.3 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianPromoterCMVAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TAL3590
Plasmid#47309DepositorInsertZebrafishCommunity-yjefn3-left (si:ch211-212d10.3 Zebrafish)
UseT7Tags3X Flag and WT FOKIExpressionMammalianPromoterCMVAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK384.4
Plasmid#155369PurposedCas9 + PA4-mVenusDepositorInsertsdCas9 (bacteria)
PA4-mVenus
UseCRISPRExpressionBacterialMutationD10A H840A (catalytically inactive)AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available SinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSE901
Plasmid#91709PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistanceDepositorTypeEmpty backboneExpressionPlantAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhU6 and hUbCAvailable SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH-ePPE
Plasmid#183097PurposeFor plant prime editing in rice plantsDepositorInsertNls-nCas9-NC-nls-MLV-Nls
UseCRISPRExpressionPlantMutationD10A in SpCas9Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorInsertdSaCas9
TagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5M-ADAM10
Plasmid#31717DepositorAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only