We narrowed to 2,455 results for: GEM

1. pESUMO-hRORg2

   Plasmid

   #170329

   Purpose

   Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

   Depositor

   Patrick Griffin

   Article

   Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

   Insert

   RORG isoform 2 (RORC Human)
   

   Use

   Tags

   HisSUMO

   Expression

   Bacterial

   Mutation

   codon optimized

   Promoter

   T7

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL3664

   Plasmid

   #162240

   Purpose

   Transcribes E. coli SRP RNA sequence for SHAPE-Seq

   Depositor

   Julius Lucks

   Article

   Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

   Inserts

   E. coli SRP RNA
   HDV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-Akt2(K14A)

   Plasmid

   #86624

   Purpose

   Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt2(K14A) (AKT2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-X1-Neo-NDUFB4

   Plasmid

   #139840

   Purpose

   Lentiviral expression of human NDUFB4

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   NDUFB4 (NDUFB4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

   Plasmid

   #52878

   Purpose

   Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E90R, D156N, T159C

   Promoter

   CaMKIIa

   Available since

   July 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

   Plasmid

   #238372

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cmlc2 (myl7)

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol1-Spe-KpnI-ubb-nmKate

   Plasmid

   #238373

   Purpose

   Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

   Depositor

   Juan Manuel González-Rosa

   Article

   Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

   Insert

   nmKate
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   ubiquitin

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACYCDuet-1:ChrA-C63S-C66S

   Plasmid

   #225082

   Purpose

   Expresses precursor peptide ChrA-C63S/C66S in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA-C63S/C66S
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C63S/C66S

   Promoter

   T7

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYCDuet-1:ChrA-C66S

   Plasmid

   #225081

   Purpose

   Expresses precursor peptide ChrA-C66S in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA-C66S
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C66S

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYCDuet-1:ChrA-C63S

    Plasmid

    #225080

    Purpose

    Expresses precursor peptide ChrA-C63S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C63S
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C63S

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDFDuet-1:ChrH-ChrI

    Plasmid

    #225083

    Purpose

    Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TBPL1 KO 1

    Plasmid

    #200210

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 1 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TBPL1 KO 2

    Plasmid

    #200211

    Purpose

    gRNA for TBPL1 KO

    Depositor

    Sheila Teves

    Article

    Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

    Insert

    TBP1 KO 2 (Tbpl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-Ub-VPS13D

    Plasmid

    #176462

    Purpose

    expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGFP-Ub-VPS13D N3521S

    Plasmid

    #176463

    Purpose

    expressing VPS13D patient mutant N3521S in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    N3521S mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]

    Plasmid

    #168168

    Purpose

    Expression of YFP-CaCyclOp(A-2x) in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    YFP-CaCyclOp(A-2x)
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    E497K and C566D

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]

    Plasmid

    #168171

    Purpose

    Expression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    YFP-CaCyclOp(A-2x)
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fuw CBX8 quad

    Plasmid

    #121118

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

    Insert

    CBX8 (R19A,R20A, R22A, W32A) (CBX8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    R19A, R20A, R22A, W32A

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. rs210-major

    Plasmid

    #113377

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 major allele (A)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2104047 major allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. rs210-minor

    Plasmid

    #113378

    Purpose

    evaluate FOXA1 DIV motif enhancer activity in SNP rs2104047 minor allele (G)

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    rs2104047 minor allele
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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