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pLPC hRap1-FL

Plasmid

#12542

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 (TERF2IP Human)

Use

Retroviral

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pMito-HyPerGRt

Plasmid

#173197

Purpose

HyPer7 and tdTomato fusion to detect mitochondrial hydrogen peroxide

Depositor

Huiwang Ai

Article

Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

Insert

Hyper7-tdTomato

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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LRRFIP2

Plasmid

#21152

Depositor

Junying Yuan

Article

Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

Insert

LRRFIP2

Tags

HA

Expression

Mammalian

Available Since

May 21, 2009

Availability

Academic Institutions and Nonprofits only
pSL157

Plasmid

#252941

Purpose

Label Escherichia coli with mCherry

Depositor

Caroline Ajo-Franklin

Article

Li et al Nat Biotechnol (2026).

Insert

mCherry

Expression

Bacterial

Promoter

PJ23101

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122

Plasmid

#223186

Purpose

Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Inserts

YAP5SA (Yap1 Mouse)
mir122 (Mir122 Mouse)

Use

AAV

Tags

HA and engineered P2A

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-deltaU6-tRNA-Phe(GAA) 1-4

Plasmid

#254428

Purpose

Mammalian expression plasmid encoding human tRNA-Phe(GAA) isodecoder 1-4.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

tRNA-Phe (anticodon GAA) 1-4

Use

Lentiviral

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shtRNA-Phe(GAA)-1

Plasmid

#254426

Purpose

Mammalian expression plasmid encoding an shRNA targeting tRNA-Phe(GAA).

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

tRNA-Phe (anticodon GAA) 1-4

Use

Lentiviral

Promoter

U6

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pSIN4-HCK

Plasmid

#254425

Purpose

Mammalian expression plasmid encoding human HCK fused to a 3_FLAG tag.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

HCK (HCK Human)

Use

Lentiviral

Tags

3_FLAG

Promoter

EF-1alpha

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-M-sgMettl1-3

Plasmid

#254418

Purpose

Mammalian expression plasmid encoding an sgRNA targeting mouse Mettl1.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

Mettl1 (Mettl1 Mouse)

Use

Lentiviral

Promoter

U6

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pMIRNA1-METTL1-msg3-WT

Plasmid

#254420

Purpose

Mammalian expression plasmid encoding human METTL1 (sgMETTL1-3-resistant) fused to a 3_FLAG tag.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

3_FLAG

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pMIRNA1-METTL1-msg6-WT

Plasmid

#254421

Purpose

Mammalian expression plasmid encoding human METTL1 (sgMETTL1-6-resistant) fused to a 3_FLAG tag.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

3_FLAG

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-M-sgMettl1-4

Plasmid

#254419

Purpose

Mammalian expression plasmid encoding an sgRNA targeting mouse Mettl1.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

Mettl1 (Mettl1 Mouse)

Use

Lentiviral

Promoter

U6

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pMIRNA1-METTL1-WT

Plasmid

#254424

Purpose

Mammalian expression plasmid encoding human METTL1 fused to a 3_FLAG tag.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

3_FLAG

Promoter

CMV

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shtRNA-Phe(GAA)-2

Plasmid

#254427

Purpose

Mammalian expression plasmid encoding an shRNA targeting tRNA-Phe(GAA).

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

tRNA-Phe (anticodon GAA) 1-4

Use

Lentiviral

Promoter

U6

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-SynaptoTAG2

Plasmid

#175275

Purpose

AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

EGFP-Synaptobrevin-2; tdTomato (Vamp2 )

Use

AAV

Tags

EGFP and tdTomato (P2A cleavage)

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCR4_miniFXN_7

Plasmid

#234360

Purpose

Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.

Depositor

Marek Napierala

Article

Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

Insert

FXN (FXN Human)

Use

Cloning

Tags

Flag Tag

Mutation

There is a partial deletion in the 5'UTR of …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-MbPylRS(DiZPK)

Plasmid

#91706

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

Depositor

P. Chen

Article

Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

Inserts

pylS
pylT

Expression

Mammalian

Mutation

L274A, C313S

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pA-His6-Tn5

Plasmid

#171871

Purpose

Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Protein A and hyperactive Tn5 transposase

Tags

His-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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phASCL1-N106

Plasmid

#31781

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

achaete-scute complex homolog 1 (ASCL1 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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CE-8e-SpRY

Plasmid

#188913

Purpose

A-to-G base editor

Depositor

Xu Ma

Article

Cao et al Mol Ther Nucleic Acids. 2022 May 4;28:73

Insert

nSpRYCas9 ecTadA-8e

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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