164,826 results

1. FUGW-PK-hLC3

   Plasmid

   #61460

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PTK2 gRNA (BRDN0001146703)

   Plasmid

   #75544

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PTK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PTK2 (PTK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPCX cyto Grx1-roGFP2

   Plasmid

   #64975

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

   Plasmid

   #100157

   Purpose

   Expresses MUS81

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   MUS81 (MUS81 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV hSyn FLEx-FRT hM4Di-mCherry

   Plasmid

   #161576

   Purpose

   Expression of hM4Di-mCherry in a Flp-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM4Di-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   hSyn

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAp63alpha-FLAG

   Plasmid

   #27008

   Depositor

   David Sidransky

   Insert

   TAp63alpha (TP63 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOZITX-S1

   Plasmid

   #184925

   Purpose

   Expresses the catalytic S1 subunit of OZITX (EcPltAB)

   Depositor

   Jonathan Javitch

   Article

   Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

   Insert

   codon optimized S1 subunit of OZITX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDZ149

   Plasmid

   #41951

   Depositor

   Stephen Elledge

   Article

   Zhang et al Cell. 2006 Aug 11;126(3):529-42.

   Insert

   ubiquitin specific peptidase 28 (USP28 )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C171A

   Promoter

   Available Since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO_FOXA1_#1

    Plasmid

    #70095

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-IRES-GFP/CALR ins5

    Plasmid

    #214701

    Purpose

    Mammalian expression of human CALR ins5

    Depositor

    Norio Komatsu

    Article

    Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

    Insert

    CALR ins5 (CALR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-NES-KIRIN1

    Plasmid

    #107416

    Purpose

    Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    KIRIN1
    

    Use

    Tags

    Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBV-Luc

    Plasmid

    #71414

    Purpose

    To see the activity of HBV transcription using Luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

    Insert

    HBV core promoter, enhancer I, enhancer II and luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HBV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227898

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227899

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJ806

    Plasmid

    #39312

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMBEC6

    Plasmid

    #187598

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ404-DogCatcher-sfGFP

    Plasmid

    #171930

    Purpose

    Express DogCatcher-superfolder GFP protein in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    DogCatcher-sfGFP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET41sIII_GST-ALC1

    Plasmid

    #173932

    Purpose

    Bacterial expression of ALC1

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    ALC1 (CHD1L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Beta-2-adrenergic receptor-mRFP

    Plasmid

    #67145

    Purpose

    Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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