We narrowed to 11,808 results for: KIN

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qTAG-C-Solo-mStayGold-H3C2

Plasmid

#227334

Purpose

Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-miRFP670nano3-H3C2

Plasmid

#227335

Purpose

Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG-HA-Regnase-3 D252N

Plasmid

#222664

Purpose

Lentiviral vector to express mouse Regnase-3 D252N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

SFFV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

Plasmid

#208170

Purpose

Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution

Tags

His-tag, SUMO-tag, and Strep-tag

Expression

Bacterial

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-ARMS1-ProLink2-MRGPRX3

Plasmid

#213962

Purpose

Expresses MRGPRX3 in mammalian cells

Depositor

Christa Müller

Article

Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

Insert

MRGPRX3 (MRGPRX3 )

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Camta2+exon minimal CMV pCDNA5

Plasmid

#212049

Purpose

LR vector for integration of Camta2+exon(21nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Camta2+exon(21nt) (Camta2 Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kansl3-exon minimal CMV pCDNA5

Plasmid

#212052

Purpose

LR vector for integration of Kansl3-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kansl3-exon (Kansl3 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kansl3+exon minimal CMV pCDNA5

Plasmid

#212053

Purpose

LR vector for integration of Kansl3+exon(78nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kansl3+exon(78nt) (Kansl3 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kdm1a+exon minimal CMV pCDNA5

Plasmid

#212055

Purpose

LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a+exon(12nt) (Kdm1a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Chtop+exon minimal CMV pCDNA5

Plasmid

#212091

Purpose

LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop+exon(138nt) (Chtop Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE FL NAP1 STOP C' EGFP IRES Blast

Plasmid

#179102

Purpose

Expresses untagged full length NAP1

Depositor

Alicia Pickrell

Article

Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

Insert

Nak associated protein 1 (AZI2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-N'-FLAG-HA-NAP1S318A

Plasmid

#195410

Purpose

Expresses FLAG-HA tagged human NAP1 with S318A point mutation

Depositor

Alicia Pickrell

Article

Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

Insert

Nak associated protein 1 (AZI2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Serine 318 turned to Alanine

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pWR16

Plasmid

#202711

Purpose

integrating bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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CMV EGFP-PPM1H-H153D

Plasmid

#200432

Purpose

Mammalian expression of H153D mutation in PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H

Tags

EGFP

Expression

Mammalian

Mutation

H153D

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA

Plasmid

#191686

Purpose

For knocking p2a-tdTomato into PTPRO locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

PTPRO homology arms and p2a-tdTomato

Expression

Mammalian

Mutation

WT

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K

Plasmid

#187274

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Mutation

Anillin A740D, E758K

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX neo PFKL-GFP, K272R

Plasmid

#138290

Purpose

Mammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP, K272R, fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

PFKL (PFKL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K272R

Promoter

CMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-caspase-1/4b

Plasmid

#183394

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of human caspase-1 fused to human caspase-4 (CASP1 Human, Synthetic)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2EFMUT

Plasmid

#174333

Purpose

encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

NKD2132DFD134>AFA (substitutions of D132 and D…

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

Plasmid

#167199

Purpose

puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 K478R/479R S364L

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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