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pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

Plasmid

#63139

Purpose

Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

Depositor

Titia Sixma

Publication

Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

Inserts

Ring1b (Ring1 Mouse)
Bmi1 (Bmi1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-109 and Residues 1-159

Promoter

tac

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMU-TGNVHAr

Plasmid

#61178

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Hes5

Plasmid

#61536

Purpose

Expresses Hes5 in mammalian cells

Depositor

Rudolf Jaenisch

Publication

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes5 (Hes5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
CRY2olig-mCh-CLC

Plasmid

#60035

Purpose

Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

Depositor

Chandra Tucker

Publication

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2-mCh-Clathrin Light Chain (CRY2 Mouse, Mustard Weed)

Expression

Mammalian

Mutation

M354I, E490G CRY2, A9D*

Promoter

CMV

Available since

Oct. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Publication

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA SF-HERC2 F4

Plasmid

#55787

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

Depositor

David Chan

Publication

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
HA-tag

Plasmid

#55182

Purpose

HA-tag

Depositor

Thorsten Mascher

Publication

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

HA tag

Use

Synthetic Biology; Bacillus biobrick box

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mAzami Green-N1 (mAG)

Plasmid

#54798

Purpose

Localization: N1 Cloning Vector, Excitation: 492, Emission: 505

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Karasawa et al J Biol Chem. 2003 Sep 5. 278(36):34167-7

Type

Empty backbone

Tags

mAzami Green

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pVITRO-HPV66 L1L2

Plasmid

#52602

Purpose

Expresses HPV66 L1 and L2 in mammalian cells

Depositor

Richard Roden

Publication

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV66 L1
HPV66 L2

Expression

Mammalian

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-synP-FLEX-splitTVA-EGFP

Plasmid

#52474

Purpose

Expresses splitTVA-EGFP

Depositor

Ian Wickersham

Publication

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Insert

splitTVA-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin-1

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-ms-Sox7

Plasmid

#50780

Purpose

retroviral expression of mouse Sox7

Depositor

Shinya Yamanaka

Publication

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox7 (Sox7 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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SeeSaw Reporter (SSR1.0)

Plasmid

#50392

Purpose

SSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Publication

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SSR1.0

Expression

Mammalian

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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TN-XXL pcDNA3

Plasmid

#45797

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Publication

Mank et al Nat Methods. 2008 Sep;5(9):805-11. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TN-XXL

Tags

ECFP and YFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBluescript-mouse eIF2C2

Plasmid

#50354

Purpose

Mouse eIF2C2 cDNA clone

Depositor

Kumiko Ui-Tei

Publication

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C2 (Ago2 Mouse)

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-G9-5FLAG-hphMX6

Plasmid

#49185

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G9-5FLAG

Expression

Yeast

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Publication

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Miro1 S432N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGoldenGate-SE9

Plasmid

#47677

Purpose

Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.

Depositor

Jose Dinneny

Publication

Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

Type

Empty backbone

Use

Arabidopsis transformation

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-ERK2

Plasmid

#47573

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged ERK2

Depositor

Kevin Janes

Publication

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

ERK2 (Mapk1 Rat)

Tags

3xFlag and GST

Expression

Bacterial

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pattB-DSCP-QF#7-hsp70

Plasmid

#46133

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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