We narrowed to 14,951 results for: RING;
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Plasmid#87550PurposePI(WT): Photo-inhibitable/blue light sensitive Rac1DepositorAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only
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pBabe-PI(WT)-Rac1(Q61L)-mCherry
Plasmid#87553PurposePI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1DepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationQ61LPromoterCMVAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP MPS1 K-R
Plasmid#59819PurposeAllows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.DepositorInsertMps1 (TTK Human)
TagsEmGFPExpressionMammalianMutationKD, catalytically inactive, D664APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [D1_n-1] (GB1208)
Plasmid#75409PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [D1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY LC3A
Plasmid#89290PurposeFor expression of the mammalian Atg8 protein LC3A without a tagDepositorAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G
Plasmid#194461PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRV-RB
Plasmid#106374Purposecreating stable line with RFP and BSD resistanceDepositorInsertmRFP and blasticidin resistance gene
UseRetroviralExpressionMammalianAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G
Plasmid#194463PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
S1 1029 CP ABEMax strategy 1
Plasmid#135357PurposeExpression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminusDepositorInsertABEMax-nSpyCas9 aa 1029
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SV40P-Tet1 enhancer E
Plasmid#63880PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 enhancer fragment E
UseLuciferaseExpressionMammalianPromoterSV40Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/BirA/FLAG-DDX54
Plasmid#97063PurposeExpresses BirA/FLAG-tagged DDX54 in mammalian cellsDepositorAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
Plasmid#46941PurposeCodes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with TALEN TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded TALEN TS from 163-218, truncated 25 amin…PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
CD8-dCherry-FRB
Plasmid#100739PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)DepositorInsertCD8A (CD8A Human)
TagsFRB and dCherryExpressionMammalianMutationDark mCherry (K70N) non-fluorescentPromoterCMVAvailable SinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [n] (GB1207)
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRV-GN
Plasmid#106373Purposecreating stable line with GFP and G418 resistanceDepositorInserteGFP and geneticin/G418 resistance gene
UseRetroviralExpressionMammalianAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL2119 pARF7 GG0-GG1
Plasmid#195910PurposeLevel 0 pARF7 promoter with GG0 and GG1 golden gate linkersDepositorInsertpARF7 (NPH4 Mustard Weed)
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G
Plasmid#194459PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM
Plasmid#107163PurposeExpresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzymeDepositorInsertF37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
TagsGSTExpressionBacterialPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:EDLL:tNos (GB1190)
Plasmid#75404PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional ActivatorDepositorInsertdCas:EDLL
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only