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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAJM.336

Plasmid

#108528

Purpose

LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LacIAM + PTac-YFP

Expression

Bacterial

Mutation

LacI Q18M A47V F161Y

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Publication

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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VinTS-tCRMod-GGSGGS9

Plasmid

#111765

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

Depositor

Brenton Hoffman

Publication

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DO(FAS)-GCaMP6s

Plasmid

#110135

Purpose

Cre-off expression of GCaMP6s

Depositor

Bernardo Sabatini

Publication

Markowitz et al Cell. 2018 May 10. pii: S0092-8674(18)30

Insert

GCaMP6s

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Publication

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENN.AAV.CAG.tdTomato.WPRE.SV40

Plasmid

#105554

Purpose

AAV expression of tdTomato from CAG promoter

Depositor

James M. Wilson

Has service

AAV1

Insert

tdtomato

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#108912

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Publication

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has service

AAV9

Insert

ChroME-ST

Use

AAV

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDisplay-R-iGluSnFR1

Plasmid

#107335

Purpose

Red fluorescent glutamate sensor based on cpmApple. Membrane-displayed

Depositor

Robert Campbell

Publication

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

R-iGluSnFR1

Expression

Mammalian

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mTagBFP2

Plasmid

#96935

Purpose

Retroviral plasmid containing mTagBFP2 for labeling of cells

Depositor

Lee Grimes

Publication

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Tag Blue Fluorescent Protein

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PresentER-Cassette GFP

Plasmid

#102942

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

Depositor

David Scheinberg

Publication

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFUSEss-CHIg-mG2a_M18

Plasmid

#105930

Purpose

Expression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Publication

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b heavy chain (Ighg2b Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCSN068

Plasmid

#101749

Purpose

Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Publication

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Publication

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A

Plasmid

#102611

Purpose

Control lentivector expressing Puro resistance and gene of interest from CMV promoter

Depositor

Nicolas Manel

Publication

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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CMV-ShadowY

Plasmid

#104621

Purpose

Expresses ShadowY in mammalian cells

Depositor

Hideji Murakoshi

Publication

Murakoshi et al Sci Rep. 2017 Jul 28;7(1):6791. doi: 10.

Insert

ShadowY

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pQP-iRIS

Plasmid

#102584

Purpose

Regulation of protein localization (near-infrared-blue-light-inducible shuttle)

Depositor

Vladislav Verkhusha

Publication

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FlagETR3 (CELF2)

Plasmid

#96900

Purpose

expression of Flag-tagged human CELF2 (aka CUGBP2)

Depositor

Thomas Cooper

Publication

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human CELF2 cDNA (CELF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDB016

Plasmid

#99220

Purpose

Accessory plasmid for aaRS PACE; negative selection

Depositor

David Liu

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII-neg

Expression

Bacterial

Promoter

PT7

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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BCL2L1_pLX307

Plasmid

#98323

Purpose

Lentiviral expression of BCL2L1

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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