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pVITRO1-BFP-NLS

Plasmid

#184458

Purpose

Nucleus-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-3xNLS

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-miR-21 toehold switch

Plasmid

#184756

Purpose

Expresses miR-21 sensor with RFP output

Depositor

Allen Liu

Article

Wang et al ACS Synth Biol. 2019 May 17;8(5):1079-10

Insert

Red Fluorescence Protein

Tags

miR-21 toehold switch

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA KanR neo

Plasmid

#167909

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_TMC4_OE_1

Plasmid

#161652

Purpose

Overexpresses TMC4

Depositor

Mike McManus

Insert

TMC4 (TMC4 Human)

Tags

V5

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-NLS-pHlu

Plasmid

#129320

Purpose

pH measurement, nucleus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

pHluorin (from H. Imamura)

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-OptoCAR-mScarlet

Plasmid

#170463

Purpose

Expresses an optically-controllable chimeric antigen receptor that is dependent on dimerizer drug, fused to mScarlet fluorophore

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

OptoCAR

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)TK2ALuc2/CMV/WPREdU3

Plasmid

#163965

Purpose

Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

HSV-1 TK and FLuc

Use

Lentiviral

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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UBC1cer120G

Plasmid

#161017

Purpose

ubiquitously weakly expressed 120-meric GFP nanocage

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

120meric GFP nanocage

Expression

Plant

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

Plasmid

#108849

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

nMag

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2143

Plasmid

#104356

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-smCBA-EmGFP-WPRE

Use

AAV

Promoter

smCBA

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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LbCas12a-DR-GC-swap-cr in pBCSK+

Plasmid

#126643

Purpose

Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat GC Swap crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109As-gfp

Plasmid

#123143

Purpose

An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 hSynapsin1 NAPA-N2 SV40

Plasmid

#97212

Purpose

FRET probe; extracelllular synapse targeted mScarlet

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

extracellular membrane tethered fluorescent protein

Use

AAV

Promoter

hSynapsin1

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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POM42-mTFP1

Plasmid

#69852

Purpose

N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1 minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pU6_T7_INT[6xminimal_Puf1]

Plasmid

#107608

Purpose

Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter

Depositor

John Rinn

Article

Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

Insert

6x minimal Puf1 motif

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSH1

Plasmid

#90295

Purpose

Expresses the fat-7 gene in C. elegans intestine

Depositor

Anne Brunet

Article

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

fat-7 (fat-7 Nematode)

Tags

No

Expression

Worm

Promoter

ges-1

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2839

Plasmid

#87765

Purpose

Prgef-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in neurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Prgef-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Prgef-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2840

Plasmid

#87764

Purpose

Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pmec-4

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2838

Plasmid

#87759

Purpose

Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmyo-3-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pmyo-3

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8-DJ1-WT

Plasmid

#29354

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Gateway Cloning

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only