We narrowed to 21,328 results for: KIN

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pMKO.1 puro-luc (shRNA)

Plasmid

#153962

Purpose

Expresses a shRNA against firefly luciferase

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

shRNA against firefly luciferase (19-bp-long target)

Use

Luciferase, RNAi, and Retroviral

Tags

N/A

Expression

Mammalian

Promoter

U6 promoter

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcFLAG-CSK-R107E

Plasmid

#74503

Purpose

Overexpression of CSK R107E mutant in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

R107E, K222R

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pMIG-Flag-RIPK1-D324A

Plasmid

#102678

Purpose

retroviral expression plasmid for RIPK1-D324A

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

Flag and IRES-GFP

Expression

Mammalian

Mutation

CHANGE Glutamate 324 to Alanine

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT sfGFP 150TAG

Plasmid

#154767

Purpose

plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p53-(1-393)-E285K-N268D

Plasmid

#35089

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His and Thrombin cleavage site

Expression

Bacterial

Mutation

Contains aa 1-393 with N269D and E285K mutations

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-TPD52

Plasmid

#172452

Purpose

Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD52 (TPD52 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGAM1_sgRNA2

Plasmid

#201615

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PGAM1 (PGAM1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-eGFP-F1C

Plasmid

#211457

Purpose

Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation blocking peptide (F1C)

Depositor

Linda DeGraffenried

Article

McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

Insert

Flotillin-1-palmitoylation blocking peptide (FLOT1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

None

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C K80D

Plasmid

#184743

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, K80D

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-PGC1(1-180)

Plasmid

#1028

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains only aa1-180 (the EcoRI site)

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pGL421_MLL_promoter

Plasmid

#118695

Purpose

Express MLL/KMT2A promoter for luciferase assay

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

MLL_promoter

Use

Luciferase

Promoter

MLL/KMT2A promoter

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_ERBB2

Plasmid

#187776

Purpose

MAC-tagged gene expression of human ERBB2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ERBB2 (ERBB2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET19b-MED17

Plasmid

#15412

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED17 (MED17 Human)

Tags

His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
EGFP-uTEV1-ACBD5

Plasmid

#171008

Purpose

HiLITR protease with ACBD5 C-terminal targeting information (peroxisome)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-ACBD5(tmd)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A][S227A]-FLAG

Plasmid

#199323

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 AT1AR-TSTS/4A

Plasmid

#45634

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-TSTS/A (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

Thr332, Ser335, Thr336, and Ser338 all changed to…

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
BRD4 CRISPRi plasmid

Plasmid

#154890

Purpose

Human BRD4 CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

BRD4-targeting gRNA

Use

CRISPR and Lentiviral

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP 5'CMV Δtat

Plasmid

#101347

Purpose

tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta Tat

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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3582 pcDNA3 Bad S136E

Plasmid

#8800

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S136E

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only