We narrowed to 4,801 results for: pir

1. MBP-MEK1 S222TAG

   Plasmid

   #68302

   Purpose

   N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-MEK1 S218TAG

   Plasmid

   #68301

   Purpose

   N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   MBP-MEK1 S218TAG (MAP2K1 Synthetic, Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. JG1202: CAG-human dLbCpf1(D832A)-NLS-3xHA-P65

   Plasmid

   #104566

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to p65 activation domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-p65
   

   Use

   Tags

   3x HA and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

   Plasmid

   #104568

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-DmrA(x1)
   

   Use

   Tags

   3x FLAG, 3x HA, and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

   Plasmid

   #104569

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-DmrA(x2)
   

   Use

   Tags

   3x FLAG, 3x HA, and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. C-His-GvpC-Calpain

   Plasmid

   #153295

   Purpose

   Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Calpain-sensitive Gas vesicle protein C (his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   1) Contains an extra glycine after the methionine…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. delNC C-His Ana GvpC in pET28a

   Plasmid

   #85736

   Purpose

   Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (truncated and his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Deletion of N and C terminal regions, addition of…

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAP14

   Plasmid

   #186262

   Purpose

   wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   wgaAB bicistronic
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCC…

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

   Plasmid

   #104570

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-DmrA(x3)
   

   Use

   Tags

   3x FLAG, 3x HA, and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Use

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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