161,398 results
-
Plasmid#163493PurposeDirect-expressing SYFP2 AAV VirusDepositorInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBetaGlobinAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα13-LgB106
Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorInsertGα13-LgB106 (GNA13 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74-irFP
Plasmid#159592PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-HA-GASC1
Plasmid#24182PurposeMammalian expression of human JMJD2C with HA tagDepositorInsertGASC1 (KDM4C Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2_SEPT9 i4
Plasmid#71617Purposemammalian expression of human SEPT9 i4 with a GFP fusionDepositorInsertSeptin 9 (SEPTIN9 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-ACE2-his expression plasmid
Plasmid#158089PurposeExpresses ACE2 in mammalian cellsDepositorInsertACE2 (ACE2 Human)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AKAR4
Plasmid#61620PurposeLipid raft-targeted FRET biosensor for monitoring PKA activityDepositorInsertLyn-AKAR4
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLCS105
Plasmid#209358PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC22A5
Plasmid#132048PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC22A5 (SLC22A5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUFlip-floxed2A-mRFP-; gcry1:BFP -1
Plasmid#173887PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; gcry1: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0481
Plasmid#142638PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHIF1A (HIF1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3518
Plasmid#144994PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNHLH2 (NHLH2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_C9orf72:1-481
Plasmid#210987PurposeMammalian expression of full-length human C9orf72. C-terminal HaloTag.DepositorInsertC9orf72:M1-F481 (C9orf72 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2-10xUAS-Voltron
Plasmid#119039PurposeUAS-driven expresion of Voltron in zebrafishDepositorInsertVoltron
UseZebrafish expressionTagsExpressionMutationPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo
Plasmid#128453Purposelentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance geneDepositorInsertEf1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-GFP
Plasmid#220198Purposemini-Tn7 GFP expression vector - LacI/pLac promoterDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterLacI/pLacAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC9A2
Plasmid#132222PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC9A2 (SLC9A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGGF_RT_FAST_RFP
Plasmid#136979PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker RFP
UseTagsExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…PromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
Plasmid#125235PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only