We narrowed to 7,249 results for: alp

1. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

   Plasmid

   #192666

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

   Plasmid

   #112215

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mutation

   D10A,H840A,N863A,C710S

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pALPS_3'LTR GFP@gag start SFFV rev

   Plasmid

   #101339

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized rev

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP

   Plasmid

   #157951

   Purpose

   Lentiviral expression of HBG site 1 sgRNA

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pALPS_3'LTR GFP@gag start SFFV tat

   Plasmid

   #101338

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized tat

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, tat
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

   Plasmid

   #79031

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(African green monkey pygerythrus)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3_alpha1_LIR1-35S-eGFP-T35S-SIR-LIR2 (GB4312)

   Plasmid

   #225435

   Purpose

   BeYDV LIR1 + TU 35S:eGFP:T35S + BeYDV SIR-LIR2.

   Depositor

   Diego Orzaez

   Insert

   LIR1:35S:eGFP:T35S-SIR:LIR2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

   Plasmid

   #225437

   Purpose

   Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.

   Depositor

   Diego Orzaez

   Insert

   CBS-att-OCS-BeYDVRep/RepA-tNos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Anti-Alpha-2C adrenergic receptor scFv [N330A/80 scFv]

   Plasmid

   #219492

   Purpose

   Mammalian Expression Plasmid of anti-Alpha-2C adrenergic receptor (Human) scFv. Derived from hybridoma N330A/80.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-Alpha-2C adrenergic receptor (Homo sapiens) recombinant mouse scFv. (ADRA2C Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211844

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter

    Plasmid

    #211847

    Purpose

    Plant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degron

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    35S

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro

    Plasmid

    #202800

    Purpose

    Lentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFalpha (with intron)

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

    Plasmid

    #196877

    Purpose

    Neuron-specific expression of mOrange2 reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

    Plasmid

    #196874

    Purpose

    Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynGFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-Inducible Human Caspase-9 Alpha C287A Mutant

    Plasmid

    #194709

    Purpose

    Inducible Expression of Human Caspase-9 Alpha C287A Mutant

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    Human Caspase-9 C287A Mutant (CASP9 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine 287 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro

    Plasmid

    #185450

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal YBBR and Strep tag in mammalian cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

    Insert

    Nucleocapsid (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    Strep-Tag II and YBBR

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

    Plasmid

    #160628

    Purpose

    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S, 35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

    Plasmid

    #141367

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

    Plasmid

    #141383

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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