4,233 results

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pFREE

Plasmid

#92050

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Expression

Bacterial

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_EGFPligand

Plasmid

#79129

Purpose

Lentiviral vector for surface expressed EGFP (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

EGFP surface ligand

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCBC-DT1T2

Plasmid

#50590

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T1, U626t plus, U629p, T2

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-HP1alpha

Plasmid

#17652

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 alpha (CBX5 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Article

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

Plasmid

#124428

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq) T2A mCherry

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.PI.Null.bGH

Plasmid

#105536

Purpose

empty AAV vector with TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

None

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 DN

Plasmid

#12680

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 25 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pSI-Check2-hRluc-NFkB-firefly

Plasmid

#106979

Purpose

Luciferase reporter with internal control for NFkB signal.

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

NFKB recognition sites (NFKB1 Human)

Use

Luciferase

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP1-10

Plasmid

#68715

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

GFP1-10

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-EGFP-WPREpA

Plasmid

#84469

Purpose

pAAV-RAM-EGFP

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

EGFP

Use

AAV

Promoter

TRE

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.LifeAct-GFP.W

Plasmid

#51010

Purpose

Lentivector that expresses LifeAct-GFP from internal human PGK promoter

Depositor

Rusty Lansford

Insert

LifeAct-GFP

Use

Lentiviral

Tags

LifeAct

Expression

Mammalian

Promoter

human PGK

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-mGFP-Actin

Plasmid

#21948

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

betaActin (ACTB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only

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Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TetOx13-CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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