Addgene

We narrowed to 2,075 results for: PAM

Showing: 1711 - 1740 of 2075 results

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pCB6HindIII-rat FcRn

Plasmid

#121671

Purpose

Expresses rat neonatal Fc receptor in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat neonatal Fc receptor (Fcgrt Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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NSarQ27G-VMAn-FKBP

Plasmid

#70230

Purpose

Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

alpha Sarcin (N-terminal portion)

Use

Tags

FK506- and rapamycin-binding protein (FKBP), Link…

Expression

Mammalian

Mutation

Changed Gln 27 to Gly

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pACEBac1-GCP2

Plasmid

#178068

Purpose

Insect cell expression of GCP2

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP2 (TUBGCP2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRibo-Tt

Plasmid

#154124

Purpose

Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

engineered bacterial rRNAs operon under control of pL promotor
bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptac and pL

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Use

Tags

Expression

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pPBpuro-LDR

Plasmid

#173857

Purpose

Encoding a part of the rapamycin-induced dimerization system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

a Lyn11-targeted FRB (LDR)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJK413

Plasmid

#155372

Purpose

dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJK429

Plasmid

#155376

Purpose

PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPS833

Plasmid

#1358

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G21V, stabilizes the GTP-bound form

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-P4:gs:nls:FKBP

Plasmid

#135984

Purpose

Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the FKBP domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-FRB-HA no-pA

Plasmid

#82595

Purpose

3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-FRB-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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3XFLAG-NSarQ27G-VMAn-FKBP

Plasmid

#70229

Purpose

Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

Depositor

Perry Howard

Article

Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

Insert

alpha Sarcin (N-terminal portion)

Use

Tags

3XFLAG, FK506- and rapamycin-binding protein (FKB…

Expression

Mammalian

Mutation

Changed Gln 27 to Gly

Promoter

CMV

Available since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pAGH51

Plasmid

#107255

Purpose

HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

HU-FRB

Use

Synthetic Biology

Tags

FRB

Expression

Mutation

Promoter

native HU promoter

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Use

Tags

His8

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Stargazin-EBFP2-FRB

Plasmid

#222417

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

Cacng2 (Cacng2 Mouse)

Use

Tags

EBFP2-HA-FRB

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-LAP2beta

Plasmid

#222416

Purpose

To express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

LAP2beta (Tmpo Rat)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-BAF

Plasmid

#222414

Purpose

To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

BAF (BANF1 Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-PML peptide

Plasmid

#175247

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

PML (PML Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PD30

Plasmid

#164543

Purpose

Expresses Drd1-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Dopamine receptor D1 (Drd1 Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-Tev

Plasmid

#84555

Purpose

MESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev protease

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FKBP-20-Tev

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-Vmn1r89

Plasmid

#133416

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 89

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r89 (Vmn1r89 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC S431A T432A NS1

Plasmid

#101837

Purpose

drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431A T432A with p0050 promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

S431A T432A

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC S431E T432E NS1

Plasmid

#101836

Purpose

drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kaiBC S431E T432E with p0050 promoter

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

S431E T432E

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-FRB(KTF)-HA no-pA

Plasmid

#82596

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Use

Tags

Expression

Mutation

W2101F

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS835

Plasmid

#8933

Depositor

Pamela Silver

Article

Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

Insert

GSP1 (GSP1 Budding Yeast)

Use

Tags

myc

Expression

Yeast

Mutation

G21V, stabilizes the GTP-bound form

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pJK412

Plasmid

#155371

Purpose

dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK430

Plasmid

#155377

Purpose

PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus
PphlF_A2NT in dCas9 sgRNA oscillator v1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-NLuc

Plasmid

#31181

Depositor

David Piwnica-Worms

Article

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge