We narrowed to 3,021 results for: PHI-1

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AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

Plasmid

#171963

Purpose

Donor plasmid for CLTX T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a-tm CD3z CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMega-MaFRSA

Plasmid

#200226

Purpose

The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

Depositor

Alanna Schepartz

Article

Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

Inserts

Pyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA

Tags

none

Expression

Bacterial

Mutation

mutated Asparagine 166 to Alanine and Valine 168 …

Promoter

proK-lacO and tacI

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

Plasmid

#136660

Purpose

Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9
ABE7.10

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and hSt1Cas9

Expression

Mammalian

Promoter

CAG and U6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a ITAM CAR

Plasmid

#171964

Purpose

Donor plasmid for CLTX CD32a-ITAM CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR

Plasmid

#171965

Purpose

Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CD3z CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00004

Plasmid

#166721

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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TA0474_pET21a(+)

Plasmid

#13839

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

IS200 transposase (Ta0474 Thermoplasma acidophilum)

Expression

Bacterial

Mutation

See Depositor Comments Below

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 A

Plasmid

#11324

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation A from figure 4A of paper.

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pMVS-V1

Plasmid

#183094

Purpose

Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

Depositor

Lars Angenent, Bastian Molitor

Article

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Inserts

pME2001
thermostable neomycin phosphotransferase gene

Use

Bacterial propagation and conjugative

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-4Glu-iRFP670

Plasmid

#167588

Purpose

Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

CPSF6-4Glu-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Mutation

K547E, R549E, R559E, R561E

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJC-mhc2.4kb-TALE3-VP64-P10

Plasmid

#104611

Purpose

Expresses TALE3 under the control of a 2.4kb mhc enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

2.4kb mhc enhancer element (Mhc Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Tags

OneSTrEP-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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LZF97_hSyn-spine-jRGECO1a

Plasmid

#119198

Purpose

Targets jRGECO1a to spines in living neurons.

Depositor

Don Arnold

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Synthetic, Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

Human Synapsin 1

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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CapChR1_pUAST-attb

Plasmid

#188040

Purpose

Expresses CapChR1 in Drosophila

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1

Tags

none

Expression

Insect

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

UAS

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0865

Plasmid

#177084

Purpose

MoClo Level 1, position 6, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase (CrG8H) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0808

Plasmid

#177082

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

tryptophan decarboxylase (CrTDC) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0863

Plasmid

#177081

Purpose

MoClo Level 1, position 5, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase (CrSLS1) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0794

Plasmid

#177073

Purpose

MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol 8-oxidase (CrG8H) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0787

Plasmid

#177072

Purpose

MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase (CrGES) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0871

Plasmid

#177087

Purpose

MoClo Level 1, position 2, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase (CrSLS1) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSquid_AA

Plasmid

#148229

Purpose

Insect Expression of DmSquid

Depositor

Elisa Izaurralde

Insert

DmSquid (sqd Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 B

Plasmid

#11325

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation B from figure 4A of paper.

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 D

Plasmid

#11327

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation D from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 C

Plasmid

#11326

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation C from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 I

Plasmid

#11332

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation I from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 E

Plasmid

#11328

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation E from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 F

Plasmid

#11329

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation F from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 G

Plasmid

#11330

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation G from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK let7 H

Plasmid

#11331

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation H from figure 4A of paper.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only