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pCMX-Gal4-TEAD4

Plasmid

#33105

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD4 (TEAD4 Human)

Tags

Gal4

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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Halo-OPTN

Plasmid

#187900

Purpose

Mammalian cell expression of Halo-tagged OPTN

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

OPTN (OPTN Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCx55.5-TrpR

Plasmid

#86373

Purpose

for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

TrpR

Use

Zebrafish

Promoter

cx55.5

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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p160Tol2-her4.3:switchCas9

Plasmid

#227680

Purpose

Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

loxP-dsRed-loxP-Cas9-t2A-GFP
4xU6:sgRNA

Use

CRISPR

Promoter

U6 and her4.3

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2

Plasmid

#27415

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pZH82 pET28a-Rai1

Plasmid

#231651

Purpose

Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

rai1 (RAI1 Budding Yeast)

Expression

Bacterial

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pZH23 Dual G-less containing ySSA1 polyA

Plasmid

#231647

Purpose

yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

SSA1 polyA termination region

Use

Synthetic Biology

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pZH20 Dual G-less containing yCYC1 polyA

Plasmid

#231646

Purpose

yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

CYC1 polyA termination region

Use

Synthetic Biology

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Pediculus humanus corporis PINK1 (115 - 575)

Plasmid

#110750

Purpose

Bacterial expression of PhPINK1 (biochemistry)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Tags

N-His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP2a-Halo

Plasmid

#175309

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP2 (IGF2BP2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK2-3xFLAG

Plasmid

#233895

Purpose

lentiviral overexpression vector for NADK2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK2 (NADK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pICSL90002

Plasmid

#68261

Purpose

Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter: U6-26 (Arabidopsis thaliana)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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CAG:: mCherry-2a-hM4Dnrxn

Plasmid

#52523

Purpose

Co-expresses hM4Dnrxn and a red fluorescent label

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

mCherry-2a-hM4Dnrxn

Tags

2xHA

Expression

Mammalian

Promoter

CAg

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-SHMT2-3xFLAG

Plasmid

#233891

Purpose

lentiviral overexpression vector for SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

SHMT2 (SHMT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pZH61 pLIB-8His3C-spt4

Plasmid

#231649

Purpose

express S.cerevisiae spt4 in insect cells

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Insert

spt4 (SPT4 Budding Yeast)

Tags

8His-HRV3C

Expression

Insect

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGem-PgpdA-Cre-TtrpC

Plasmid

#168784

Purpose

Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Promoter

PgpdA

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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JB82

Plasmid

#128911

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab10TN-BirA*-HA-MAO (RAB10 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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JB81

Plasmid

#128910

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab10QL-BirA*-HA-MAO (RAB10 Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Dronpa-Lifeact-7

Plasmid

#54740

Purpose

Localization: Actin, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Article

Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

Insert

Lifeact-Dronpa

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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