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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Publication

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Publication

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Has service

AAV8

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Promoter

EFS

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pCMV5-Flag-XBP1s

Plasmid

#63680

Purpose

Mammalian expression of Flag tagged XBP1s

Depositor

Qingbo Xu, Lingfang Zeng

Publication

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

spliced

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

Plasmid

#51085

Purpose

Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has service

AAV1 and AAV Retrograde

Insert

GCaMP6f

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Promoter

human Synapsin1

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pmTurquoise2-H2A

Plasmid

#36207

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

H2A (H2AC16 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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p212 pCMV-EGFP/RFP

Plasmid

#8389

Purpose

Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

Depositor

Jeffrey Green

Publication

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP

Use

Cre/Lox

Expression

Mammalian

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Skp2

Plasmid

#19947

Depositor

Yue Xiong

Publication

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Tags

myc

Expression

Mammalian

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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EGFP-hAgo2

Plasmid

#21981

Depositor

Phil Sharp

Publication

Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Argonaute 2 (AGO2 Human)

Expression

Mammalian

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pBAD/myc-his A Rv0712 (FGE)

Plasmid

#16132

Purpose

Bacterial expression of Mycobacterium tuberculosis FGE

Depositor

Carolyn Bertozzi

Publication

Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Available since

Nov. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3-TLR2-YFP

Plasmid

#13016

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 2 (TLR2 Human)

Tags

YFP

Expression

Mammalian

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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SPLICS Mt-ER Short P2A

Plasmid

#164108

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Publication

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Short P2A

Expression

Mammalian

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Publication

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pHM6-alphasynuclein-WT

Plasmid

#40824

Depositor

David Rubinsztein

Publication

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

6x His and HA

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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SRa-HA-SUMO2 (Sentrin 3)

Plasmid

#17360

Purpose

Mammalian expression of human SUMO2 with HA tag

Depositor

Edward Yeh

Publication

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO2 (SUMO2 Human)

Tags

HA

Expression

Mammalian

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMSCV-FlagMLL-pl-ENL(5613)

Plasmid

#20873

Depositor

Robert Slany

Publication

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL (KMT2A, MLLT1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pFlag-CMV1-hMD2

Plasmid

#13028

Depositor

Doug Golenbock

Insert

MD2 (LY96 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCAG-YFP

Plasmid

#11180

Purpose

Mammalian expression vector for expression of YFP

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

EYFP from Aequorea victoria

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLXSN16E6E7

Plasmid

#52394

Purpose

The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

Depositor

Denise Galloway

Publication

Halbert et al J Virol. 1991 Jan;65(1):473-8. doi: 10.1

Insert

HPV16 E6E7

Use

Retroviral

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pHM6-alphasynuclein-A53T

Plasmid

#40825

Depositor

David Rubinsztein

Publication

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

A53T

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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