164,826 results

1. Flag-ZDHHC21

   Plasmid

   #231669

   Purpose

   Expresses human ZDHHC21protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC21 (ZDHHC21 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HBM-Luc

   Plasmid

   #35155

   Depositor

   Linda Penn

   Article

   Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

   Insert

   Myc promoter (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains 2.5 kb of the c-myc minimal promoter

   Promoter

   c-myc

   Available Since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMYC-GFP

   Plasmid

   #42142

   Depositor

   Olufunmilayo Olopade

   Article

   Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

   Insert

   c-Myc (MYC Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-Solo-mStayGold-VIM

   Plasmid

   #227302

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   VIM Homology Arms flanking a mStayGold Tag (VIM Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-PITCh-VIM

   Plasmid

   #227301

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of VIM (VIM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SeAvs3

   Plasmid

   #188791

   Purpose

   Avs3 from Salmonella enterica NCTC13175

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   SeAvs3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3/Myc-DNMT3A

   Plasmid

   #35521

   Purpose

   Mammalian expression of human DNMT3A with myc tag

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3A (DNMT3A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCasY1

   Plasmid

   #87687

   Purpose

   p15A plasmid expressing CasY1

   Depositor

   Jennifer Doudna

   Article

   Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

   Insert

   CasY1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTPH405c

   Plasmid

   #185726

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Promoter

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHyg-AID*-GFP

    Plasmid

    #99521

    Purpose

    C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(71-116) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p45sheLL

    Plasmid

    #37323

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV45L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

    Plasmid

    #52521

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA on ChR2

    Expression

    Mutation

    Promoter

    CBA

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE30-His-AlaRS

    Plasmid

    #124103

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Alanyl-tRNA ligase (alaS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJYDN

    Plasmid

    #162450

    Purpose

    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. phU1-CasRx-non-targeting (pKW82)

    Plasmid

    #229050

    Purpose

    Cloning backbone for CasRx crRNA (Human U1 promoter)

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR-Cas9-ScaligD

    Plasmid

    #125688

    Purpose

    Gene knockout/in for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spCas9, sgRNA casette, and ScaligD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 TFAM-mClover

    Plasmid

    #129574

    Purpose

    Expression of mClover fused TFAM

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. kiCAP-AAV-MyoAAV3F

    Plasmid

    #224447

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDHASW insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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