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GFP-GW1D1b(aa502-751)

Plasmid

#21521

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa502-751

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pST39-His/Vps27(345-440) - Hse(275-375)

Plasmid

#17638

Depositor

James Hurley

Article

Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

Insert

His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV L3-L2

Plasmid

#62092

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 L3-L2

Plasmid

#62096

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV R4-R3

Plasmid

#62093

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE77

Plasmid

#80579

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-VHL-Flag(117-213)

Plasmid

#29501

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Tags

Flag and His

Expression

Insect

Mutation

VHL(117-213) only

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pET16b-Trx80

Plasmid

#36945

Depositor

Anna Blom

Article

King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

Insert

Trx80 (truncated Trx) (TXN Human)

Tags

His tag

Expression

Bacterial

Mutation

premature stop inserted after K80 in Trx1 gene

Promoter

T7

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
pBJ1 neo-mo FceRI beta

Plasmid

#8604

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI beta cDNA (Ms4a2 Mouse)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-ZO-2-1st-PDZ

Plasmid

#27424

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL G457D

Plasmid

#61367

Purpose

Expresses Human full length RIPK3 with G457Dmutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with G457D mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed G457 to D

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL N464D

Plasmid

#61369

Purpose

Expresses Human full length RIPK3 with N464D mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with N464D mutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed N464to D

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pFA6a-GFP (F64L, S65T, V163A)-His3MX6

Plasmid

#53185

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

HIs3MX6 (HIS3 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pEF5/FRT-DEST EVC2ala4-Flag

Plasmid

#41008

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed amino acids 1183-1186 to Alanine

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only
NHA-Ago3m

Plasmid

#21532

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago3 (AGO3 Human)

Tags

GFP

Expression

Mammalian

Mutation

missing aa757-823, the C-termianl 66aa of PIWI do…

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only
GST-GW1D12(aa895-1211)

Plasmid

#21528

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GST

Expression

Mammalian

Mutation

contain aa895-1211

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
GST-GW1D1(aa254-751)

Plasmid

#21523

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GST

Expression

Mammalian

Mutation

contain aa254-751

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only