164,826 results

1. pCMV-GFP-TFE3pA

   Plasmid

   #120201

   Purpose

   Human TFE3 poly(A) site reporter

   Depositor

   David Bartel

   Article

   Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

   Insert

   TFE3 poly(A) site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

   Plasmid

   #222994

   Purpose

   Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Puro-mStayGold-LMNB1

   Plasmid

   #227329

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-CBE6d

   Plasmid

   #215822

   Purpose

   Express CBE6d (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6d-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pV1393:SpRY

   Plasmid

   #172189

   Purpose

   Genome editing of C. albicans

   Depositor

   Doug Bernstein

   Article

   Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PTK2 gRNA (BRDN0001146703)

   Plasmid

   #75544

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PTK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PTK2 (PTK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CTV

   Plasmid

   #15912

   Depositor

   Klaus Rajewsky

   Article

   Thai et al Science. 2007 Apr 27. 316(5824):604-8.

   Insert

   CAG-STOP-eGFP-ROSA26TV
   

   Use

   Cre/Lox; /stop

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-I-SceI

   Plasmid

   #60960

   Purpose

   Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   I-SceI restriction enzyme
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. plenti-ITGB6-HA

   Plasmid

   #235242

   Purpose

   Lentiviral vector to generate HA-tagged human ITGB6 stable expressing cell line

   Depositor

   Jan Carette

   Article

   Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

   Insert

   ITGB6 (ITGB6 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIN4-CMV-K2M

    Plasmid

    #21164

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Klf4, cMyc (MYC, KLF4 Human)
    

    Use

    Lentiviral; Reprogramming

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHA-VRC01-scFv

    Plasmid

    #44874

    Depositor

    Dane Wittrup

    Article

    Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

    Insert

    VRC01 scFv
    

    Use

    Tags

    HA and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pECNUS4

    Plasmid

    #184887

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 Flag-humanTRAF2 (1087)

    Plasmid

    #66931

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL20_mEGFP_EWSR1_ERG

    Plasmid

    #205819

    Purpose

    Express mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    EWSR1_ERG (EWSR1, ERG Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid

    #185913

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShuttle-Flag-XBP1u

    Plasmid

    #63679

    Purpose

    Adenoviral shuttle vector for XBP1u expression

    Depositor

    Lingfang Zeng, Qingbo Xu

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Promoter

    CMV

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pTRE3G-mouse cGAS-HA

    Plasmid

    #186875

    Purpose

    Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Mouse cGAS with C-terminal HA tag (Cgas Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Str-Ii_SBP-mCherry-Golgin84

    Plasmid

    #65304

    Purpose

    synchronize trafficking of Golgin-84 from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROPseq-tRFP

    Plasmid

    #230938

    Purpose

    Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

    Depositor

    Hyejung Won

    Article

    Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBMCL1

    Plasmid

    #178203

    Purpose

    An improved vector for BacMam system

    Depositor

    Lei Chen

    Article

    Guo et al Biochem Biophys Res Commun. 2022 Feb 26;

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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