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We narrowed to 24,354 results for: crispr

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Showing: 11601 - 11620 of 24354 results

  1. pKS1617

    Plasmid
    #239548
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239548-plasmid-map-sequence-id-478367

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  2. pKS1618

    Plasmid
    #239549
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239549-plasmid-map-sequence-id-478366

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  3. pU6:3-tevopreq1

    Plasmid
    #190909
    Purpose
    Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Insert
    Empty Backbone
    Use
    CRISPR
    Expression
    Insect
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190909-plasmid-map-sequence-id-373031

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  4. pKS1619

    Plasmid
    #239550
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239550-plasmid-map-sequence-id-475997

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  5. pKS1601

    Plasmid
    #239526
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239526-plasmid-map-sequence-id-475905

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  6. pKS1531

    Plasmid
    #239521
    Purpose
    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239521-plasmid-map-sequence-id-475896

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  12. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60231-plasmid-map-sequence-id-417804

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  16. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60226-plasmid-map-sequence-id-176980

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  17. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

    Plasmid
    #223124
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223124-plasmid-map-sequence-id-447705

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  20. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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Showing: 11601 - 11620 of 24354 results