We narrowed to 30,509 results for: des.2

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pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4

Plasmid

#216822

Purpose

Expresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-Broccoli#4

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-EAMP

Plasmid

#138265

Purpose

mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

iCas9 target-mCherry

Use

CRISPR

Expression

Mammalian

Promoter

EF1a-HTLV

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pTPH405c

Plasmid

#185726

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C) single PAM linker

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5-ActRIIA

Plasmid

#11754

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

Activin receptor IIA (Acvr2a Mouse)

Expression

Mammalian

Available Since

Aug. 14, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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1318 pcDNA3 flag FKHRL1

Plasmid

#10708

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHRL1 (FOXO3 Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pAGM38869

Plasmid

#153220

Purpose

Level 0 Arabidopsis U6-promoter vector

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis U6 snRNA promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-CAAX

Plasmid

#87612

Purpose

Plasma membrane-targeted Venus (YFP).

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-Fast-1 wild-type

Plasmid

#16521

Depositor

Bert Vogelstein

Article

Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

Insert

FAST-1 (FOXH1 Human)

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
CMV-p150

Plasmid

#51412

Purpose

expression of full length of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pAC-PHYTipi

Plasmid

#53283

Purpose

Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-C1-P4Mx2

Plasmid

#202681

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

PAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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phR-Luc

Plasmid

#139644

Purpose

Mammalian gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

Renilla luciferase

Use

Luciferase

Promoter

SV40

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMD.RVG.CVS24-B2c

Plasmid

#19713

Depositor

Manfred Schubert

Article

Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

Insert

Rabies virus CVS24-B2c glycoprotein

Expression

Mammalian

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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GFAP-Cre

Plasmid

#24704

Depositor

Michael Sofroniew

Article

Bush et al Neuron. 1999 Jun . 23(2):297-308.

Insert

Cre

Expression

Mammalian

Promoter

GFAP (glial fibrillary acidic protein)

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Rho-EGFP-ETSQVAPA

Plasmid

#45399

Depositor

David Williams

Article

Trivedi et al J Neurosci. 2012 Aug 1;32(31):10587-93.

Insert

Rhodopsin (RHO Bovine)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pBP-J23100

Plasmid

#72963

Purpose

Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

J23100 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-lacZ

Plasmid

#72948

Purpose

Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 0 - Bioparts

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHes1-DN-GFP (CC#110)

Plasmid

#15134

Depositor

Connie Cepko

Article

Furukawa et al Neuron. 2000 May . 26(2):383-94.

Insert

Hes1 (Hes1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

E43A, K44A, R47A

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only