We narrowed to 57,330 results for: plasmid
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Plasmid#164094PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-mVenus
UseLentiviral and Synthetic BiologyAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GRK2-CAAX
Plasmid#166224PurposeGRK2-CAAX mammalian expression plasmidDepositorInsertbovine GRK2-CAAX (GRK2 Bovine)
TagsnoExpressionMammalianMutationadding CAAX sequence at C terminalPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorPublicationTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-crRNA-Cm
Plasmid#158711PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInserteforCP (for crRNA cloning)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterTetOAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-crRNA-Km
Plasmid#158712PurposePlasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNADepositorInsertAcGFP (for crRNA cloning)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterTetOAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5372
Plasmid#128733PurposeGateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tagDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Violet
Plasmid#160447PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Violet
UseSynthetic BiologyMutationBioBrick sites removedAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH130
Plasmid#162484PurposeSEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-URA
Plasmid#160004PurposeLow-copy expression vector for K. marxianus, uracil selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsn/aExpressionYeastPromotern/aAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
DHC1-AID-Clover donor (Neo)
Plasmid#158621PurposeDonor plasmid for tagging DHC1 with AID-CloverDepositorInsertDHC1 (DYNC1H1 Human)
UseCRISPR; Tagging donorAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
4xHRE-MinTK-CRE
Plasmid#141144Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsert4xHRE-MinTK-CRE
UseLentiviralAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Brp-T2A-QF2w-Hsp70-3XP3-dsRed
Plasmid#141094PurposeTemplate plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.DepositorInsertbruchpilot
UseCRISPRExpressionInsectMutationcoding sequence downstream of CRISPR cut site, sy…Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSup_35Senh(fwd)_35Spr+synJ
Plasmid#149422PurposePlasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.DepositorInsert35S core enhancer
ExpressionPlantAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO
Plasmid#155048PurposeLentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct-GW-HygroR
Plasmid#149610PurposeGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.DepositorInsertGateway cassette (ccdb + Chlor.R.)
ExpressionInsectPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH1
Plasmid#154204Purposeencodes CRISPR-Cas9 system derived from plasmid pCas9DepositorTypeEmpty backboneUseCRISPRAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2(C506S, L4A)
Plasmid#136580Purposemammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)DepositorInsertNRF2 (C506S, Leuzip mut) (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationThe C506S mutation (C513S in the actual sequence …Available sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCO604
Plasmid#139737Purposeindividual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnH3HDepositorInsertH3H
UseLuciferase; Moclo level 0 componentPromoternoneAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMCRW1
Plasmid#122462PurposeHuman NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cellsDepositorTagsHis TagExpressionBacterialPromoterT7lac promoterAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only