176,436 results

Showing: 5841 - 5850 of 176436 results

Results per page:

Sort results by:

pAWP78-PVCpnf1-16

Plasmid

#198271

Purpose

Unmodified pPVC; contains WT PVC structural/accessory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf1-16

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Publication

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Expression

Mammalian

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pDAC627

Plasmid

#195240

Purpose

Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Publication

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro

Expression

Mammalian

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171379

Purpose

Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Publication

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized Cas13X.1

Expression

Mammalian

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CGBE1 (pRZ3885)

Plasmid

#140252

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Publication

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
VRQR-ABEmax

Plasmid

#119811

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6 promoters

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Publication

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

Enlarge
pET_RTX_(exo-)

Plasmid

#102786

Purpose

Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

Depositor

Andrew Ellington

Publication

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase with 3'-5' deficient exonuclease

Expression

Bacterial

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge