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pPiggyBac_NTVE(PABP)-dVSVG-HA

Plasmid

#254462

Purpose

Transposon encoding doxycycline-inducible NTVE(PABP) with HA tag. Co-expression of PiggyBac transposase required. Tet-On 3G and puromycin N-acetyltransferase are co-expressed via CAG promoter.

Depositor

Gil Westmeyer

Publication

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

NTVE(PABP)_P2A_dVSV-G-HA

Expression

Mammalian

Promoter

TRE3G

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-CaBLAM_294W

Plasmid

#244130

Purpose

Lower affinity variant of CaBLAM bioluminescent calcium indicator

Depositor

Nathan Shaner

Publication

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Insert

CaBLAM_294W

Expression

Mammalian

Mutation

Calmodulin N98W

Available since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pBIG2AB-eIF3klefhdm

Plasmid

#240041

Purpose

Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d and eIF3m expression in insect cells for purification

Depositor

Venki Ramakrishnan

Publication

Diaz-Lopez et al Protein Sci. 2026 Jan;35(1):e70388. doi:

Insert

eIF3k, l, e, f, h, d, m

Tags

eIF3d: HA

Expression

Insect

Mutation

WT

Promoter

Polyhedrin

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-LATeNT

Plasmid

#240993

Purpose

Expresses LATeNT in mammalian cells; CaMKII promoter

Depositor

Alice Ting

Publication

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

V5-LATeNT

Use

AAV

Tags

V5

Expression

Mammalian

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0056_pIVTRup_dCas9-VP64

Plasmid

#242542

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Publication

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-VP64

Use

CRISPR

Mutation

D10A

Available since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCART0002_pIVTRup(BsmBI)_CBE4max-SpRY

Plasmid

#242540

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Publication

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CBE4max-SpRY (SpRY-CBE)

Use

CRISPR

Mutation

D10A

Available since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Cas9-DEN2

Plasmid

#238017

Purpose

for DNA-free gene knockout editing in rice and wheat or other plants

Depositor

Caixia Gao

Publication

Qiu et al Plant Biotechnol J. 2025 Apr;23(4):1348-

Insert

SpCas9

Expression

Plant

Promoter

T7

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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73-79 ZBneg PANCS-binder negAP

Plasmid

#240121

Purpose

ZBneg PANCS-binder negative AP

Depositor

Bryan Dickinson

Publication

Styles et al bioRxiv [Preprint]. 2025 Jan 17:2025.01.

Insert

-ZBneg-RNAPC fusion

Promoter

Custom

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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PC2328

Plasmid

#232089

Purpose

Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.

Depositor

Pranam Chatterjee

Publication

Hong et al Nat Commun. 2025 Apr 15;16(1):3555. doi:

Insert

duAb with cloning Esp3I restriction site

Expression

Mammalian

Promoter

CMV

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Publication

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-mCherry-SopF

Plasmid

#229745

Purpose

Expresses mCherry-tagged SopF in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Publication

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

mCherry-SopF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pZZMP09

Plasmid

#231754

Purpose

Mammalian expression of SPO11

Depositor

Scott Keeney

Publication

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11 (Spo11 Mouse)

Expression

Mammalian

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-MCS-WPRE-SV40 - GCH13

Plasmid

#230820

Purpose

AAV cloning vector for expression under the EF1a promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV; Cloning vector

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS853

Plasmid

#228236

Purpose

Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible EYFP reporter for integration into Bxb1 attP_GA landing pad

Depositor

Arvind Rasi Subramaniam

Publication

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Inserts

mCherry
EYFP

Tags

HA and PuroR

Expression

Mammalian

Promoter

TRE3GV and none

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-ADE (V5 and HA)

Plasmid

#222489

Purpose

Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Publication

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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His_CL7_2NLS_iCas12a-4NLS

Plasmid

#222971

Purpose

Bacterial expression of NLS-rich iCas12a construct

Depositor

Jennifer Doudna

Publication

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His_CL7_2NLS_iCas12a-4NLS

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-SpyTag003

Plasmid

#216282

Purpose

Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SUMO-SpyTag003

Tags

His6 and SpyTag003

Expression

Bacterial

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-G88P3-HA-hM3Dq

Plasmid

#213972

Purpose

AAV vector with G88P3 promoter that can drive robust gene expression in MSNs

Depositor

Zhonghua Lu

Publication

Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

Insert

hM3D (Gq)

Use

AAV

Promoter

G88P3

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pR6K-GFP-CASTIF

Plasmid

#199653

Purpose

R6K plasmid encoding a type I-F CAST but no CRISPR array

Depositor

IIya Finkelstein

Insert

CAST I-F systems

Expression

Bacterial

Promoter

J23119 promoter

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Publication

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Expression

Mammalian

Promoter

SFFV

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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