We narrowed to 28,961 results for: Tat

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pBABE_hygro_mRFP1_NRF2(C506S, L4A)

Plasmid

#136580

Purpose

mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (C506S, Leuzip mut) (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Mutation

The C506S mutation (C513S in the actual sequence …

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-PAMmut-Hygro

Plasmid

#176086

Purpose

EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutation in the PAM site used by PolBKOg1

Promoter

CMV

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS-TDP-43

Plasmid

#162614

Purpose

Allows for transcription of improved mVenus I152L fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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px335 Mettl14 sgRNA #2

Plasmid

#61514

Purpose

encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

Depositor

Jacob Hanna

Article

Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

Insert

gccgctcccggatctcctgc

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLgw ELK4-V5-EcoDam

Plasmid

#98607

Purpose

Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK4 (Elk4 Mouse)

Use

Lentiviral; Damid

Tags

V5 and Dam (DNA adenine methyltransferase)

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CCL2_WT_V5

Plasmid

#82935

Purpose

Gateway Donor vector containing CCL2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CCL2 (CCL2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.G524C

Plasmid

#82779

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Mutation

G524C; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCW57.1 KBTBD4 P311PP

Plasmid

#184625

Purpose

Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 P311PP expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal Flag tagged KBTBD4 P311PP (KBTBD4 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

P311PP

Promoter

TRE promoter, Tet ON

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_CGREF1_WT

Plasmid

#82895

Purpose

Gateway Donor vector containing CGREF1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CGREF1 (CGREF1 Human)

Use

Gateway entry vector

Mutation

deletion of AA 233-249

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 2)

Plasmid

#99562

Purpose

mammalian expression of TMEM230-GFP (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

Plasmid

#156452

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG

Depositor

Elphege Nora

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

RAD21, AID (Rad21 Mouse)

Use

Mouse Targeting

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP43 M337V

Plasmid

#162612

Purpose

Allows for transcription of mVenus fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Mutation

M337V

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-fd [Chronos-GFP]

Plasmid

#84482

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SEPT_Cdk2-T160A-siR

Plasmid

#73975

Purpose

HDR template to generate Cdk2-T160A-siR

Depositor

Steven Dowdy, Manuel Kaulich

Article

Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

Inserts

Cdk2-LHA-siR (CDK2 Human)
Cdk2-RHA-T160A (CDK2 Human)

Use

AAV

Mutation

T160 mutated to Ala and siRNA target site mutated

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.T790M

Plasmid

#82848

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway entry vector

Mutation

T790M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 associated agonist of cell death (BAD Human)

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1-S15/25A

Plasmid

#52364

Purpose

expresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

S15A, S25A and R95Q (according to numbering in Fi…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 R312RG

Plasmid

#184630

Purpose

Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)

Expression

Mammalian

Mutation

R312RG

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E453Q

Plasmid

#82844

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway entry vector

Mutation

E453Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3a

Plasmid

#180432

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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