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pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)

Plasmid

#160619

Purpose

Module for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

ERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-ITSN1

Plasmid

#176117

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDECKO_mCherry_non-targeting

Plasmid

#140109

Purpose

CRISPR-Cas9 library validation (negative control)

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Promoter

gRNA1 under U6 and gRNA2 under H1

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSI-359

Plasmid

#131131

Purpose

gRNA expression vector for A-to-G base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

A-to-G reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

Plasmid

#123194

Purpose

binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL030

Plasmid

#137899

Purpose

STU2.0 SpyCas9 Csy4 system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRSpuro shID1#6

Plasmid

#19165

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pLV Gpat4-V5

Plasmid

#175145

Purpose

Lentiviral expression of mouse Gpat4-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Gpat4 (Gpat4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

Plasmid

#118702

Purpose

lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

Flag

Mutation

wt , SLC7A11 sh926R

Available Since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-ASAP2s

Plasmid

#101275

Purpose

Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

AAV

Expression

Mammalian

Promoter

CAMKIIa

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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TRP1MX6-ins4

Plasmid

#195041

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

TRP1

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.E2.ChETA

Plasmid

#220310

Purpose

Directs ChETA optogenetic actuator to fast spiking interneurons in cortex. Allows for single APs to be evoked.

Depositor

Matt Rowan

Article

Kumar et al Nat Commun. 2024 Apr 1;15(1):2823. doi:

Insert

ChETA

Use

AAV

Expression

Mammalian

Promoter

S5E2

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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KG#205

Plasmid

#110881

Purpose

Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

changed Asp 448 to Asn

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad4

Plasmid

#89829

Purpose

Knockdown human Smad4 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

Smad4 shRNA (SMAD4 Human, Mouse)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGEL033

Plasmid

#137902

Purpose

STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET21b-rrt-His8

Plasmid

#194088

Purpose

Expresses Ec86 reverse transcriptase in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 rrt

Tags

His8

Promoter

T7

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf ctnna1L344P

Plasmid

#185407

Purpose

Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

ctnna1 (ctnna1 Zebrafish)

Use

In vitro synthesis of mrna

Expression

Mammalian

Mutation

changed Leucine 344 to Proline

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV/myc/ER-αT2ib

Plasmid

#110769

Purpose

Expresses ER intrabody against mouse and human TLR2

Depositor

Thomas Böldicke

Article

Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

Insert

intrabody against mouse and human TLR2

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-XS-VWF

Plasmid

#64847

Purpose

Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

Depositor

Sriram Neelamegham

Article

Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

Insert

Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)

Tags

7X His, Cerulean, and Venus

Expression

Bacterial

Promoter

T7

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (91-536)

Plasmid

#42206

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (91-536) (SRC Human)

Expression

Mammalian

Mutation

contains amino acids 91-536

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only