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SEPLuc-E2A-cyOFP1

Plasmid

#141084

Purpose

precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-E2A-cyOFP1

Use

Lentiviral

Promoter

CMV

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R]

Plasmid

#140064

Purpose

Mammalian Expression Plasmid of anti-KCNT1/Slo2.2/Slack K+ channel (Rat). Derived from hybridoma N3/26.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-KCNT1/Slo2.2/Slack K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnt1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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sg1

Plasmid

#113966

Purpose

Single short guide RNA targeting GTATAGCATACATTATACG

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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sg2

Plasmid

#113967

Purpose

Single short guide RNA targeting TACCACATTTGTAGAGGTT

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg2

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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sg3

Plasmid

#113968

Purpose

Single short guide RNA targeting CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg3

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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sg2+sg3

Plasmid

#113970

Purpose

Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg2+sg3

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)

Plasmid

#134291

Purpose

Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acids 30, 280 and 443 to Asparag…

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v2

Plasmid

#134277

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 2)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

553 amino acids, isoform 2

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK (281-473)

Plasmid

#134273

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 281-473

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK (12-272)

Plasmid

#134272

Purpose

Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk amino acids 12-272

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-N-Flag-GK_v1

Plasmid

#134270

Purpose

Lentivector encoding Flag-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk isoform 1; 524 amino acids

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-005 Lyn-Neptune//BsaI

Plasmid

#130186

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Lyn-Neptune

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-010 LifeAct-Neptune//BsaI

Plasmid

#130191

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

LifeAct-Neptune

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-015 Vimentin-Neptune//BsaI

Plasmid

#130196

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

Vimentin-Neptune

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI

Plasmid

#130197

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-Sirius

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-017 NLSNoLS-SuperNova//BsaI

Plasmid

#130198

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-SuperNova

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-VALENTIA-033 NLSNoLS-Eos//BsaI

Plasmid

#130214

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

NLSNoLS-Eos

Use

Synthetic Biology

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-615 C-Tag Venus210[1] L20 Stat3(R609Q) EBPF2

Plasmid

#130090

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-614 N-Tag Venus210[1] L10 Stat3(R609Q) EBPF2

Plasmid

#130089

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Expression

Mammalian

Mutation

R609Q

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-611 C-Tag Venus210[2] L32 Stat3 mCherry

Plasmid

#130086

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3 (Stat3 Mouse)

Expression

Mammalian

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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