162,326 results

1. pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized

   Plasmid

   #172345

   Purpose

   Expression of FLAG-tagged, codon-optimized, full-length SRSF3

   Depositor

   Sujatha Jagannathan

   Article

   Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

   Insert

   serine and arginine rich splicing factor 3 (SRSF3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   EF-1a

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCGN N-Ras wt

   Plasmid

   #14723

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Insert

   N-ras (NRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW TetO HA-AMPKa1 WT

   Plasmid

   #69824

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV500 A-CREB

   Plasmid

   #33371

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   CREB (Creb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1-DEST

   Plasmid

   #119749

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-KRAS

   Plasmid

   #116755

   Purpose

   Lentiviral expression of KRAS

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. acGFP NES visual barcode

   Plasmid

   #158673

   Purpose

   tagging clonal populations with NES acGFP fluorescent protein. Adding NES to acGFP WC

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   acGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA GFP10-ZIP

   Plasmid

   #182241

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP
   

   Use

   Tags

   split-GFP10 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA2219

   Plasmid

   #38152

   Depositor

   David Waugh

   Article

   Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

   Insert

   DsbC (dsbC E. coli)
   

   Use

   Tags

   N-terminal leader peptide (cloning artifact)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_502_sgCD4

    Plasmid

    #158704

    Purpose

    CD4 CRISPRa control

    Depositor

    David Root, John Doench

    Article

    Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK293 (mAID-mCherry2-Hygro)

    Plasmid

    #72831

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMyNT

    Plasmid

    #42191

    Depositor

    Matthias Wilmanns, Annabel Parret

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    ColE1

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Ii-OVA.II

    Plasmid

    #64597

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 315-353 (class II epitopes)

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKH115_GALNT2-keima

    Plasmid

    #214969

    Purpose

    Golgiphagy flux reporter GALNT2-keima

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    GALNT2-keima (GALNT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC67841

    Plasmid

    #209512

    Purpose

    Cloning in complete guide expression cassettes, with Cas12a (D156R)

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TXNRD1

    Plasmid

    #38863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TXNRD1 (Txnrd1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-mem-TurboID

    Plasmid

    #149409

    Purpose

    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    mem-TurboID
    

    Use

    Tags

    HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNLF1-C_PLCG1:1-1291

    Plasmid

    #211089

    Purpose

    Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    PLCG1:M1-L1291 (PLCG1 Human)
    

    Use

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    isoform 2. I813T natural variant (VAR_011908)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1 shRUNX1 puro

    Plasmid

    #45816

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    Runx1 (RUNX1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge