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pDONR223_ERBB2_WT

Plasmid

#82113

Purpose

Gateway Donor vector containing ERBB2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERN1_WT

Plasmid

#82115

Purpose

Gateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERN1 (ERN1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AR_WT

Plasmid

#82094

Purpose

Gateway Donor vector containing AR , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AR (AR Human)

Use

Gateway Cloning

Mutation

p.81_82insQQ; deletion of AA397-482

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAi14-luc-MC

Plasmid

#87113

Purpose

minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

Luciferase-SV40pA

Use

CRISPR and Luciferase

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-P2A-CIAR_pLenti_CMV_Puro

Plasmid

#86503

Purpose

Lenti plasmid for generating CIAR expressing stable cell lines

Depositor

Dustin Maly

Publication

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

GFP-P2A-CIAR (SOS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2-HTH-HHT2

Plasmid

#86482

Purpose

yeast expression of HHT2 with His10-TEV-HA tag

Depositor

Akash Gunjan

Publication

Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

Insert

HHT2 (HHT2 Budding Yeast)

Tags

HIS10-TEV-HA (HTH)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pGEX-4T-1-Skp2 WT

Plasmid

#81117

Purpose

bacterial expression plasmid of Skp2

Depositor

Sung Hee Baek

Publication

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

SKP2 (SKP2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only
non-targeting control gRNA (BRDN0001149423)

Plasmid

#80255

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148500)

Plasmid

#80260

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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BAS292 C terminal SF-GFP kanR

Plasmid

#74087

Purpose

S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

Depositor

Bassem Al-Sady

Publication

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

SF-GFP recoded for S.pombe

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pNR291

Plasmid

#79555

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

Depositor

Timothy Lu

Publication

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

GFPmut3b
mTagBFP
mRFP

Expression

Bacterial

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-Plk4

Plasmid

#80264

Purpose

baculovirus creation plasmid for GST-Plk4

Depositor

Andrew Holland

Publication

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

GST

Expression

Insect

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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PRKDC gRNA (BRDN0001145772)

Plasmid

#77862

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKDC

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKDC (PRKDC Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK1 gRNA (BRDN0001149093)

Plasmid

#77788

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK1 (MAPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MLKL gRNA (BRDN0001149268)

Plasmid

#77541

Purpose

3rd generation lentiviral gRNA plasmid targeting human MLKL

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MLKL (MLKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT2 gRNA (BRDN0001148949)

Plasmid

#77506

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT2 (AKT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BRD4 gRNA (BRDN0001147232)

Plasmid

#77345

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRD4 gRNA (BRDN0001145474)

Plasmid

#77347

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD4

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD4 (BRD4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001148950)

Plasmid

#77193

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001148866)

Plasmid

#77191

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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