We narrowed to 2,325 results for: PUC19

1. pcloDF-amp

   Plasmid

   #184844

   Purpose

   Backbone that confers Ampicillin resistance, contains cloDF ori

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPR

   Plasmid

   #186276

   Purpose

   Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira shortened CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR

   Plasmid

   #165043

   Purpose

   Repair template for CAS9 complex genome editing

   Depositor

   Brian Chen

   Article

   Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

   Insert

   RPL13A (RPL13A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pILGFP1H5

   Plasmid

   #165065

   Purpose

   Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFPY2A

   Plasmid

   #165066

   Purpose

   Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pISKP1-TIR1

   Plasmid

   #165061

   Purpose

   Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pILGFP105

   Plasmid

   #165060

   Purpose

   Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pILGFP104

   Plasmid

   #165059

   Purpose

   Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJAID9L104

   Plasmid

   #165054

   Purpose

   Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC_cherry donor_Rosa target (pE002)

    Plasmid

    #149348

    Purpose

    Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC_cherry donor_Polb target (pE038)

    Plasmid

    #149349

    Purpose

    Replace donor vector for cherry insertion using the human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJBL7007

    Plasmid

    #136946

    Purpose

    Malachite green aptamer with stability hairpin, bacterial promoter

    Depositor

    Julius Lucks

    Article

    Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

    Insert

    malachite green aptamer (MGA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZNF445-donor

    Plasmid

    #112332

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF445 with GFP

    Depositor

    Kevin White

    Insert

    ZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs78585428, hg38:3:44446218:C>T, hg38:3:444466…

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZNF785-donor

    Plasmid

    #112317

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF785 with GFP

    Depositor

    Kevin White

    Insert

    ZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:16:30582292:A>T, hg38:16:30582302:A>T,…

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAP1071-2

    Plasmid

    #99502

    Purpose

    3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas73

    Plasmid

    #82398

    Purpose

    Δccm:Km, used to delete ccm operon

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    ccm deletion
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-Mb-crRNA

    Plasmid

    #78959

    Purpose

    Cloning vector for expression of MbCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAD03 S. bayanus telomerase RNA KBS

    Plasmid

    #61892

    Purpose

    Plasmid for run-off in vitro transcription of Saccharomyces bayanus KBS RNA

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pAD05 S. castellii telomerase RNA KBS

    Plasmid

    #61894

    Purpose

    Plasmid for run-off in vitro transcription of Saccharomyces castellii KBS RNA

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAD02 S. paradoxus telomerase RNA KBS

    Plasmid

    #61891

    Purpose

    Plasmid for run-off in vitro transcription of Saccharomyces paradoxus KBS RNA

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only