We narrowed to 13,789 results for: crispr grnas

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Construct 10 - U6p_40nt_stgRNA

Plasmid

#81249

Purpose

Expresses 40nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA_expression_vector

Plasmid

#210212

Purpose

An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

Depositor

Albert Jeltsch

Article

Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGH224_sgRNA_2xMS2_Puro

Plasmid

#85413

Purpose

hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p206_LTJ_sgRNACD45.2_R3

Plasmid

#82674

Purpose

sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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M-NM-sgRNA

Plasmid

#48673

Purpose

Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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p184_LTJ_sgRNACD90.2

Plasmid

#82670

Purpose

sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD90.2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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M-SP-sgRNA

Plasmid

#48671

Purpose

Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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XX>sgRNA F+E

Plasmid

#87543

Purpose

Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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p183_LTJ_sgRNACD90.2_A

Plasmid

#82671

Purpose

sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD90.2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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EC0_6_sgRNA

Plasmid

#163717

Purpose

Plasmid encoding a placeholder sequence as a Type 234 part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

sgRNA expression cassette

Use

Synthetic Biology; Moclo vector level 0 (type 234)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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p240_LTJ_sgRNAFoxp3K276X

Plasmid

#82675

Purpose

sgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting Foxp3 K276X

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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p204_LTJ_sgRNACD45.2_R2

Plasmid

#82673

Purpose

sgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD45.2 region 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p163_LTJ_sgRNACD90.1

Plasmid

#82677

Purpose

sgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD90.1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

Plasmid

#196847

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

Plasmid

#196846

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

Plasmid

#196844

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

Plasmid

#196845

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

Plasmid

#120293

Purpose

AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

N-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

SV40-NLS and split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

Plasmid

#50946

Purpose

An empty gRNA expression vector

Depositor

Kosuke Yusa

Article

Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2014

Availability

Academic Institutions and Nonprofits only

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p426-SNR52p-gRNA.csr-1.Y-SUP4t

Plasmid

#68060

Purpose

gRNA for csr-1 locus in N. crassa

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gRNA for csr-1 locus

Use

CRISPR; N. crassa, fungi

Expression

Yeast

Promoter

SNR52

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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