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Plasmid#235839PurposepetA 5'UTR Chlamydomonas reinhardtiiDepositorInsertpetA 5'UTR
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_3_005_petB_5'UTR_Cr
Plasmid#235833PurposepetB 5'UTR Chlamydomonas reinhardtiiDepositorInsertpetB 5'UTR
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_1_007_petB_5'Hom_cr
Plasmid#235787Purpose5' Homology petB in the M1 position used for genome integration at the petB locusDepositorInsert5' Homology region of the petB locus of Chlamydomonas reinhardtii chloroplast CC-125
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-AvrPWT4
Plasmid#237522PurposeRecombinant protein expressionDepositorInsertAvrPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-RWT4Ca
Plasmid#237519PurposeRecombinant protein expressionDepositorInsertRwt4Ca
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-MBP-KD
Plasmid#237520PurposeRecombinant protein expressionDepositorInsertRwt4_kinase
UseTagsExpressionBacterialMutationKinase region of RWT4PromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:GST-VirPWT4
Plasmid#237523PurposeRecombinant protein expressionDepositorInsertVirPwt4
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec holD-pET(3c)
Plasmid#237236Purposeover-express E. coli holD (Pol III psi subunit)DepositorInsertholD (psi) (holD E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec holC-pET(3c)
Plasmid#237235Purposeover-express E. coli holC (Pol III chi subunit)DepositorInsertholC (chi) (holC E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6-pG-Tnp
Plasmid#124884PurposeTo transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.DepositorInsertprotein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6-pG-MNase
Plasmid#124886PurposeTo transform BL21-Gold(DE3) and be induced by IPTG for His-proteinG-MNase fusion protein expression.DepositorInsertprotein G-MNase
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-T4Dda
Plasmid#228215PurposeExpression in BL21(DE3) of T4 Dda-like helicaseDepositorInsertdda (dda Escherichia phage T4)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29b_GST-Nb32
Plasmid#228895PurposeExpress GST-tagged anti-Zika Nb32 in E.coliDepositorInsertGST-tagged Nb32
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29b_GST-NbD6
Plasmid#228896PurposeExpress GST-tagged anti-Zika NbD6 in E.coliDepositorInsertGST-tagged NbD6
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFLtail
Plasmid#232055PurposeExpresses construct for surface tethering: R. norvegicus NFL tail domainDepositorInsertNeurofilament-Light tail domain
UseTags6xHisExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
UseTags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf1
Plasmid#232058PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 1DepositorInsertNeurofilament-Medium tail domain, charge shuffle 1
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
UseTags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…PromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMshuf2
Plasmid#232059PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domain with more evenly distributed charged residues, sequence 2DepositorInsertNeurofilament-Medium tail domain, charge shuffle 2
UseTags6xHis, thrombinExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only