We narrowed to 14,026 results for: CAR
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Plasmid#184907Purposecarrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferriduransDepositorInsertCm-sgRNA-dCas9
UseCRISPRExpressionBacterialAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH136
Plasmid#47554PurposepJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.DepositorInsertURA3
UsePcr templatePromoterNative URA3 promoter, -243 to -1.Available SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN3FS-CYC1
Plasmid#85783PurposeN-ICE plasmid pH3FS-CYC1DepositorInsertloxP-NatMX-loxP-CYC1p-3FLAG
UseCre/LoxExpressionYeastMutation*(see below)Available SinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL22-sgRNA NT1
ExpressionBacterialPromoterPL22Available SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-USP13
Plasmid#61741Purposeexpress N-terminally FLAG tagged human USP13DepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_HelperΔgIII
Plasmid#190433PurposeM13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.DepositorInsertM13 genes I-V and VII-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST5552
Plasmid#36255PurposeMycobacteria replicating vector expressing eGFP.DepositorInserteGFP
UseSynthetic Biology; Mycobacteria replicating vectorExpressionBacterialPromoterModifiedHsp60Available SinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
RAP1B
Plasmid#55665Purposeexpression of mouse RAP1BDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
RAP1A
Plasmid#55664Purposeexpression of mouse RAP1ADepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197
Plasmid#67551PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2195
Plasmid#67548PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
ANO10 (TMEM16KA-c001)
Plasmid#110267PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMslc
Plasmid#32095DepositorAvailable SinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pORF1-Flag
Plasmid#190565PurposeMammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.DepositorInsertORF1 (from L1.3)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+Paxillin-mKate
Plasmid#105974Purposein vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesionsDepositorInsertPaxillin
TagsmKateExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
TagsSV40 NLSExpressionYeastPromoterTEF1 promoterAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp R4-4 mutant
Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
TagsHis tagExpressionBacterialAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB3405
Plasmid#106166PurposeEasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_CMV_MeltCasp1-37-GFP
Plasmid#234069PurposeEncodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°CDepositorInsertMeltCasp1-37-GFP
UseLentiviralExpressionMammalianMutationMelt(C292A,Q355N); hcaspase1(R391K)PromoterCMVAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only