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pSKC12 - DelQ

Plasmid

#110493

Purpose

Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'- DelQ (bp 30-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Deletion of the first 29 base pairs of the NRAS 5…

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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Mito-mTurq2-Apollo-NADP+

Plasmid

#193303

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

mTurqoise2 and mitochondrial targeting sequence

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-SF2

Plasmid

#99020

Purpose

Bacterial expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Perox-mTurq2-Apollo-NADP+

Plasmid

#193304

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

mTurqoise2, peroxisomal targeting sequence

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (FANCC)

Plasmid

#180192

Purpose

AAV vector carrying a guide RNA targeting the human FANCC mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (CTNNB1)

Plasmid

#180193

Purpose

AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX5X.1 PINK1 KD

Plasmid

#13322

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496; Kinase dead: K219D/D362A/D3…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (1-300) (P#432)

Plasmid

#14608

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-300) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-300

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
GB1-His-TET1-848-952(pL91)

Plasmid

#124388

Purpose

E.Coli produced TET1-848-952 purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-848-952 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Nuc-mTurq2-Apollo-NADP+

Plasmid

#193302

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

Depositor

Jonathan Rocheleau

Article

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Insert

G6PD (G6PD Human)

Tags

SV40 NLS and mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-EZH2-T487A mutant

Plasmid

#28061

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Tags

GST

Expression

Bacterial

Mutation

T487A

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-6p-hDcp2 E147/8Q

Plasmid

#72217

Purpose

bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

E147Q, E148Q and F404L (please see depositor'…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK7a1-EE

Plasmid

#47578

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK7a1-EE (Map2k7 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Residues 73-420 of RefSeq sequence. Ser271 to Gl…

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pETDuet-1-6xHis-TEV-Nsp8

Plasmid

#201022

Purpose

Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (1-250) (P#467)

Plasmid

#14606

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-250) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-250

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

Plasmid

#163644

Purpose

Makes a lentivirus that expresses mNeonGreen and Cas9.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

mNeonGreen-P2A-Cas9

Use

Lentiviral

Tags

mNeonGreen-P2A

Promoter

EFS

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBC2T-Ypet-FtnA

Plasmid

#108969

Purpose

expresses BC2-tag-Ypet-FtnA in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

FtnA (ftnA Escherichia coli)

Tags

BC2-tag and Ypet

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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