We narrowed to 12,205 results for: 112

1. Notch1 intracellular domain-pcw107

   Plasmid

   #64621

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   NOTCH1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Mutation

   intracellular domain *

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCjeCas9

   Plasmid

   #172215

   Purpose

   Expresses Campylobacter jejuni Cas9 (CjeCas9) in bacterial cells

   Depositor

   John van der Oost

   Article

   Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

   Insert

   Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system
   

   Tags

   Histag

   Promoter

   T7

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-PGK-hCD2

   Plasmid

   #224294

   Purpose

   Expresses sgRNA from U6 promoter and human CD2 under PGK

   Depositor

   Nikhil Joshi

   Article

   Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

   Insert

   U6 sgRNA cassette with CD2 under PGK promoter (CD2 Human)
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   U6/PGK

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SHIP2-ΔPS

   Plasmid

   #214901

   Purpose

   expression of the truncated variants of the SHIP2 without phosphatase domain

   Depositor

   Pavel Krejčí

   Article

   Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

   Insert

   human SHIP2 delta aa 419-739 (INPPL1 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   delta 419-739aa

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-sACE2v2.4(732)-8h

   Plasmid

   #154103

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-G…

   Promoter

   CMV

   Available Since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hras (G12V)-pcw107

   Plasmid

   #64603

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Mutation

   G12V

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

7. MEK5 DD(S311D,T315D)-pcw107-V5

   Plasmid

   #64619

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAP2K5 (MAP2K5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Mutation

   S311D, T315D*

   Promoter

   PGK

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

8. p38 WT O/E (MAPK14)-pcw107-V5

   Plasmid

   #64624

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   MAPK14 (transcript variant 2) (MAPK14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

9. CL20_mEGFP_PAX5_NOL4L

   Plasmid

   #205886

   Purpose

   Express mEGFP-tagged fusion protein, PAX5_NOL4L from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   PAX5_NOL4L (PAX5, C20orf112 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti GW V5 Eco/Dam hum LaminA

    Plasmid

    #182673

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.

    Depositor

    Bas van Steensel

    Article

    Meuleman et al Genome Res. 2013 Feb;23(2):270-80. doi:

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Promoter

    HSP

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CMV-TMPRSS2-TwinStrep_Zeo

    Plasmid

    #192000

    Purpose

    Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    TMPRSS2

    Expression

    Mammalian

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcdna6.2- myc ULK1 4SA

    Plasmid

    #27631

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 4SA (Myc Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S467A, S555A, T574A, S637A. Initial M from ULK1 r…

    Available Since

    March 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus-tBid-4E-pEGFP-C1

    Plasmid

    #166743

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    tBid-4E (Bid Mouse)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pαH-S-GSAS-B.1.1.7

    Plasmid

    #171747

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_LNX1_WT

    Plasmid

    #81804

    Purpose

    Gateway Donor vector containing LNX1 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    LNX1 (LNX1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid

    #196975

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    6xHis and SNAP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. px335 Mettl14 sgRNA #2

    Plasmid

    #61514

    Purpose

    encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

    Depositor

    Jacob Hanna

    Article

    Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

    Insert

    gccgctcccggatctcctgc
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. shERK2b-mlpx-puro

    Plasmid

    #65230

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE_hygro_mRFP1_NRF2(C506S, L4A)

    Plasmid

    #136580

    Purpose

    mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (C506S, Leuzip mut) (NFE2L2 Human)
    

    Use

    Retroviral

    Tags

    N-mRFP1-FLAG

    Expression

    Mammalian

    Mutation

    The C506S mutation (C513S in the actual sequence …

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2-shATRX

    Plasmid

    #124258

    Purpose

    Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shATRX
    

    Expression

    Mammalian

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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