159,589 results

1. SpCas9-CBE6c-IVT

   Plasmid

   #215835

   Purpose

   Template for in vitro transcription of CBE6c (with SpCas9)

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-SpCas9-2xUGI
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12489_PEAR-GFP_2in1_2.0

   Plasmid

   #177186

   Purpose

   PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCL-HA-MAPKK1-11A55B [S218E/S222D]

   Plasmid

   #40809

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S218E, S222D

   Promoter

   CMV

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. medSIIN

   Plasmid

   #185664

   Purpose

   shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

   Depositor

   Tyler Jacks

   Article

   Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

   Inserts

   mScarlet
   miR-30a
   shApc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p415-PtrpC-Cas9-TtrpC-CYC1t

   Plasmid

   #68059

   Purpose

   Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   humanized Cas9
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 MBP Asn10 TEV LIC cloning vector (1C)

   Plasmid

   #29654

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   10xAsn, 6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pERM2-nhpSer

   Plasmid

   #201922

   Purpose

   Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   SepRS(2)
   Sep-tRNA v2.0
   SerB
   EF-Sep
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   E412P, E414F, T417K, P495M, I496W, F529S and H67R…

   Promoter

   GlnS (constitutive), OXB20 (constitutive), lpp (c…

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-HA_WT RAD23A

   Plasmid

   #201458

   Purpose

   Expresses human RAD23A with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAJM.677

   Plasmid

   #108530

   Purpose

   AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   AraCAM + AraE + PBAD-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AraC: L133F E165L E169G C280L

   Promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQP-T2A

    Plasmid

    #102583

    Purpose

    Light-controlled activation of expression

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-VP16-T2A-NLS-GAL4-QPAS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-TAX1BP1

    Plasmid

    #171423

    Purpose

    For the purifcation of TAX1BP1 protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mPA-GFP-CD81-10

    Plasmid

    #57124

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    CD81 (CD81 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. HRE-dUnaG

    Plasmid

    #201711

    Purpose

    Hypoxia responsive promoter driving destablized UnaG fluorescent protein

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ERK-SPARK

    Plasmid

    #106921

    Purpose

    Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    ERK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEFIRES-P-ACSL3-mCherry

    Plasmid

    #87158

    Purpose

    Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ACSL3 (ACSL3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Rab5A Q79L

    Plasmid

    #28046

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab5A (RAB5A Human)
    

    Use

    Tags

    EGFP on N-term

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-FKBP-SAC1deltaTMD

    Plasmid

    #108132

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-521)

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4

    Plasmid

    #193294

    Purpose

    episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-17-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SOX2-17 is engineered highly cooperative chimeric…

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCiPS

    Plasmid

    #60011

    Purpose

    Foamy virus helper plasmid expressing Pol, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

    Insert

    Pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HBM-Luc

    Plasmid

    #35155

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 2.5 kb of the c-myc minimal promoter

    Promoter

    c-myc

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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