169,392 results
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Plasmid#218868PurposeMammalian expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
ExpressionMammalianMutationS99A F102Y F104Y L178SPromoterCAGAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XMyc-EGFP-PEX26
Plasmid#139059PurposeMyc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XMyc-EGFP-PEX26
UseLentiviralTagsMycPromoterUBCAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C-terminal GST
Plasmid#128025PurposeMammalian expression vector with C terminal GST tagDepositorTypeEmpty backboneTagsGSTExpressionMammalianAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTns(Ptr)
Plasmid#175576PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTnsABC
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH47761
Plasmid#48003DepositorTypeEmpty backboneUseUnspecifiedAvailable SinceDec. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0037
Plasmid#141488PurposeLentiviral vector for overexpressing the SP5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP118
Plasmid#105495PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusionsDepositorInsertHupA-SmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3B-Fc(DAPA)-AviTag-6xHis
Plasmid#156956PurposeMammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.DepositorInsertSEMA3B (SEMA3B Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Active Wnt5A-V5
Plasmid#43813DepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP(LaG17) PAGER(TF)
Plasmid#229997PurposeExpresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EGFR-V5/HIS
Plasmid#201102Purposeexpression of human EGFR receptor tyrosine kinase in mammalian cellsDepositorInserthuman EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-beta-arrestin2
Plasmid#36919DepositorAvailable SinceJune 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(H134R)-EYFP
Plasmid#26969PurposeAAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorHas ServiceAAV1, AAV2, AAV5, and AAV9InserthChR2(H134R)
UseAAVTagsEYFPExpressionMammalianMutationHistidine 134 is mutated to ArgininePromoterCaMKIIaAvailable SinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFD116
Plasmid#124769PurposepFD116 carries dcas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
dcas9
PpflB sgRNA BsaI
UseCRISPRAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF256
Plasmid#105496PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiTDepositorInsertHupA-SmBiT/LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCM184-ParB2::eGFP
Plasmid#87252PurposeExpresses ParB2-EGFP in yeast. Also known as pOR2-GFPDepositorInsertParB2
TagsEGFPExpressionYeastPromoterpBAD/tetOAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Expotin 1
Plasmid#42047DepositorAvailable SinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-KRAS
Plasmid#116755PurposeLentiviral expression of KRASDepositorAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-5.5kb
Plasmid#58890Purposeprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
UseGateway 5' entry cloneMutationmutated single nucleotide (T-->C, 13th nucleot…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only