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Showing: 11881 - 11900 of 169392 results

  5. mEmerald-Integrin-Alpha2-N-18

    Plasmid
    #54128
    Purpose
    Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Integrin-Alpha2 (ITGA2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. LentiV_Neo_SIK3_CR_T221A

    Plasmid
    #108107
    Purpose
    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    SIK3 (SIK3 Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    change guanine 501 to cytosine (silent mutation),…
    Promoter
    EFS promoter
    Available Since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108107-plasmid-map-sequence-id-210058

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  10. piggyFlex

    Plasmid
    #218234
    Purpose
    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.
    Depositor
    Jay Shendure
    Article
    Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    218234-plasmid-map-sequence-id-432525

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  12. pUC57-white[coffee]

    Plasmid
    #84006
    Purpose
    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
    Depositor
    Phillip Zamore
    Article
    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.
    Insert
    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    Use
    Unspecified
    Mutation
    A GC-to-AA mutation that creates a G589E missense…
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    84006-plasmid-map-sequence-id-165529

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  13. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

    Plasmid
    #205281
    Purpose
    CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.
    Depositor
    Matthias Prigge
    Insert
    GCaMP6s
    Use
    AAV
    Available Since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205281-plasmid-map-sequence-id-412571

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  15. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid
    #197572
    Purpose
    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    Tags
    none
    Expression
    Bacterial
    Mutation
    3TAG and 45TAG
    Promoter
    araC and lpp
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197572-plasmid-map-sequence-id-388086

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Showing: 11881 - 11900 of 169392 results